中国组织工程研究与临床康复2011，Vol.15Issue(2)：245-248,4.DOI:10.3969/j.issn.1673-8225.2011.02.013
筛选介导化学合成siRNA转染原代肝癌细胞的最佳试剂
Transfection agents screening for chemosynthesis siRNA transfection to primary liver cancer cells
摘要
Abstract
BACKGROUND: The ideal transfection agent should efficient and low-toxic.OBJECTIVE: To screen transfection agent which efficiently transfect chemosynthesis siRNA to primary liver cancer cells.METHODS: FAM-siRNA and MDR1 siRNA was transfected to primary liver cancer cells by Lipofectamine RNAiMAX,Lipofectamine 2000 and DharmaFECT1. The transfection efficiency was evaluated by flow cytometer and real time-PCR at 6 and 48 hours after transfection. Moreover, the cytotoxicity of transfection agents in primary liver cancer cells was tested by MTT method.RESULTS AND CONCLUSIONS: The results of flow cytometer and real time-PCR indicated that, the transfection efficiency of siRNA tranfection to primary liver cancer cells mediated by RNAiMAX was highest (P ＜ 0.05), which was 70.3％ and 71.5%,respectively. The cytotoxicity of RNAiMAX in primary liver cancer cells did not show in MTT detection (P ＜ 0.05). RNAiMAX was suitable to transfect siRNA to primary liver cancer cells because the efficiency of siRNA tranfection to primary liver cancer cells mediated by RNAiMAX was the highest and the cytotoxicity of RNAiMAX in primary liver cancer cells was the lowest.关键词
原代肝癌细胞/siRNA/转染/Lipofectamine RNAiMAX/Lipofectamine 2000/DharmaFECT1/组织工程分类
医药卫生引用本文复制引用
王玉洁,吴韶菊,吴芹..筛选介导化学合成siRNA转染原代肝癌细胞的最佳试剂[J].中国组织工程研究与临床康复,2011,15(2):245-248,4.基金项目
课题受临沂师范学院立项课题(HX10602)资助.课题名称:抗肝癌药物及其分子机制研究. （HX10602）
