中国组织工程研究与临床康复2011，Vol.15Issue(2)：196-200,5.DOI:10.3969/j.issn.1673-8225.2010.02.002
骨关节炎软骨中衰老相关β-半乳糖苷酶和c-Fos蛋白的表达
Expression of senescence-associated beta-galactosidase and c-Fos protein in osteoarthritic cartilage
李良军 1雷光华 2涂敏 2程超 2陈霞光 2高曙光 2曾凯斌2
作者信息
- 1. 长沙市中心医院骨科,湖南省长沙市,410004
- 2. 中南大学湘雅医院骨科,中南大学骨科研究所,湖南省长沙市,410008
- 折叠
摘要
Abstract
BACKGROUND :The expression of enescence-associated β-galactosidase (SA-β-gal) serves as a marker of cell senescence,while the expression of c-Fos in cartilage of osteoarthritis is rare.OBJECTIVE:To study expression differences of SA-β-gal and c-Fos protein in normal and impaired cartilage of osteoarthritis.METHODS: Ten normal cartilages and 26 cartilage of osteoarthritis were selected. On the basis of improved Mankin pathological score, 50 cartilage specimens to divide into normal, mild impaired, medium impaired and severe impaired groups. The expression of SA-β-gal and c-Fos protein in the cartilage specimens were detected by SABC method. The difference and correlation between the percentage of the positive chondrocytes and the different impaired cartilage were analyzed.RESULTS AND CONCLUSION:The cartilage degeneration aggravated with increasing of SA-beta-gal positive chondrocytes,suggesting that chondrocyte senescence has contacted with osteoarthritis. The expression of c-Fos protein shows no direct correlation with the severity of osteoarthritis and chondrocyte senescence. The findings demonstrated that chondrocyte senescence may be one of pathogenesis of osteoarthritis, which promotes compensate for cell division in the early and medium stage of osteoarthritis.关键词
骨关节炎/软骨细胞/衰老相关β-半乳糖苷酶/c-Fos蛋白/原癌基因c-fos分类
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李良军,雷光华,涂敏,程超,陈霞光,高曙光,曾凯斌..骨关节炎软骨中衰老相关β-半乳糖苷酶和c-Fos蛋白的表达[J].中国组织工程研究与临床康复,2011,15(2):196-200,5.
