应用化学2011，Vol.28Issue(1)：83-87,5.DOI:10.3724/SP.J.1095.2011.00124
滇桂艾纳香水溶性多糖BRP-B的分离纯化及止血活性
Isolation,Purification and Hemostatic Activity of Water-soluble Polysaccharide in Blumea Riparia(BI.)DC.
摘要
Abstract
Water-soluble polysaccharide B(BRP-B) in Blumea riparia(BI. ) DC. was extracted and purified by using membrane separation, discoloration on diethylaminoethyl (DEAE) -cellulose, and sephadex column chromatography of G10 and G50. Results from high-performance gel permeation chromatography (HPGPC) showed that the obtained BRP-B has a narrow-distributed molecular mass, with the number average molecular mass and mass average molecular mass as 2654 and 2716, respectively. The drug activity test based on plasma recalcification as the index showed that BRP-B with the optimal concentration of 6. 25 x 10 -2 g/L had the strongest procoagulant activity, the coagulation time was 248. 52 s, and the inhibition rate of coagulation time was 22.42％. In particularly, BRP-B with concentration of 1. 67 x 10 -2, 1. 97 x 10 -2 and 2. 85 x 10 -2 g/L could significantly enhance the frequency of uterus muscle.关键词
滇桂艾纳香/多糖/分离/纯化/止血活性分类
化学化工引用本文复制引用
廖敏富,林翠梧,黄丽,许子竞,覃飒飒,李爱媛..滇桂艾纳香水溶性多糖BRP-B的分离纯化及止血活性[J].应用化学,2011,28(1):83-87,5.基金项目
国家自然科学基金(20662001,20962002)资助项目,2007年国家大学生创新实验项目(071059314) （20662001,20962002）
