天然产物研究与开发2011，Vol.23Issue(1)：105-109,5.
固定云芝漆酶纳米金颗粒修饰金盘电极的制备及其对氧还原反应的生物电催化性能
Preparation of Gold Disk Electrode Modified by Nanogold Particle with Laccase through Covalent Coupling and Its Bioelectrocatalytic function towards Oxygen Reduction Reaction
曾涵 1龚兰新 1崔婷 1侯凤兰1
作者信息
- 1. 新疆师范大学生命科学与化学学院化学系,乌鲁木齐,830054
- 折叠
摘要
Abstract
Gold disk electrode was modified with nanogold particle using 1,6-hexanedithiol (HET) as linker and nanogold particle modified with L-cystein to be functionalized was reacted with laccase enough to immobilize enzyme tightly.After doing so,gold disk electrode modified with nanogold particle which covalently coupled with laccase has been achieved. The catalytic function towards oxygen reduction reaction (ORR) of prepared electrode was evaluated with cyclic voltammograms (CV). The results obtained from experiments which indicated dioxygen reduction reaction on prepared electrode occurred at about -0.14 V ( vs SCE), much better than that of literature [ 7 ] reported (-0.26 V vs NHE) ITO electrode modified with poly(N-isopropylacrylamide) (PNIPAM) hydrogel;peak current of ORR on prepared electrode was 3.0 μA or so,about 6.5 times of that of literature[ 7 ] reported ITO electrode modified by PNIPAM with laccase.Further investigation showed gold disk electrode modified by nanogold particle with immobilized laccase through covalent coupling had excellent stability for long-term use and had better thermal stability than literature[ 7 ] reported ITO electrode modified with PNIPAM hydrogel:prepared electrode can retain 40％ of peak current corresponding to oxygen reducfion at 25 ℃ when system temperature was kept at 50 ℃.关键词
漆酶/直接电迁移(DET)/氧还原(ORR)Key words
laccase/direct electron transfer(DET) /oxygen reduction reaction(ORR)分类
化学化工引用本文复制引用
曾涵,龚兰新,崔婷,侯凤兰..固定云芝漆酶纳米金颗粒修饰金盘电极的制备及其对氧还原反应的生物电催化性能[J].天然产物研究与开发,2011,23(1):105-109,5.
