天然产物研究与开发2011，Vol.23Issue(1)：142-145,4.
三种维药提取物对蛋白酪氨酸磷酸酶1B抑制作用的研究
Inhibitory Effect of Extracts from Three Uyghur Medicine on the Protein Tyrosine Phosphatase 1 B Activity
摘要
Abstract
Protein tyrosine phosphatase-1 B is one of new drug targets against insulin sensitizer. In this study, we investigated the inhibitory effect of petroleumether extracts from 3 Uygur herbs,including Ziziphora Clinopodioides Lam. ,Helichrysum arenarium (Lima.) Moench,and Ver-nohia anthelmintica (L.) willd,on the PTP1B activity and the mode of inhibition. The results showed that recombinant PTP1 B was highly expressed by the prokaryotic expression system and its molecular weigh was 40.8 kDa. Three extracts showed different inhibitory effects on the PTP1B activity,with IC50 values of 20.2 μg/mL, 12.65 μg/mL,and 9.7 μg/mL,respectively. The enzyme kinetic studies revealed non-competitive type of inhibition,competitive type of inhibition, and mixed competitive type of inhibition with the inhibitory constants (Ki) of 0.275 μg/mL, 1.245 μg/mL,and 0. 192 μg/mL,respectively. The study will provide support for further exploration and utilization of Uygur herbs.关键词
维药/蛋白酪氨酸磷酸酯酶1B/抑制作用/原核表达Key words
Uygur herbs /PTP1B /inhibition/ prokaryotic expression system分类
农业科技引用本文复制引用
刘禾培,窦君,马纪,信学雷,赵海清,阿吉艾克拜尔·艾萨..三种维药提取物对蛋白酪氨酸磷酸酶1B抑制作用的研究[J].天然产物研究与开发,2011,23(1):142-145,4.基金项目
中国科学院、国家外国专家局创新团队国际合作伙伴计划,新疆维吾尔自治区高技术研究与发展计划(200611109) （200611109）
国家科技支撑计划(2007BAI30B03) （2007BAI30B03）
