中国组织工程研究与临床康复2011，Vol.15Issue(1)：23-28,6.DOI:10.3969/j.issn.1673-8225.2011.01.005
脂肪来源神经干细胞移植局灶性脑缺血模型大鼠的血管新生
Angiogenesis in a rat model of focal cerebral ischemia following adipose tissue-derived neural stem cell transplantation
摘要
Abstract
BACKGROUND: Adipose tissue-derived neural stem cells transplantation may improve neurological function in rats with cerebral ischemia injury, but its mechanism remains unclear.OBJECTIVE: To study the effects of human adipose tissue-derived neural stem cells transplantation on angiogenesis in rats after focal cerebral ischemia.METHODS: Adipose tissue-derived stromal cells cultured in vitro were differentiated into neural stem cells. A total of 60 healthy male Sprague-Dawley rats were assigned into four groups randomly: normal control group (n=6), sham-operated group (n=6),ischemia control group (n=24) and transplantation treated group (n=24). Models of 2-hour ischemia/reperfusion of rat middle cerebral artery were established by suture-occluded method in the ischemia control group and transplantation treated group.lschemia control group and transplantation treated group were divided randomly into 7, 14, 21 and 28 days reperfusion groups (n=6). Middle cerebral artery was not occluded in the sham-operated group. On hour 24 following model induction, human adipose tissue-derived neural stem cell suspension (2×109 /L) was given in the transplantation treated group via the caudal vein.Saline was injected into the rats in ischemia control group via the caudal vein. Immunohistochemistry was used to identify microvessel density (MVD). The blood vessel proliferation in the rat focal cerebral ischemia area was observed.RESULTS AND CONCLUSION : Immunohistochemistry results have exhibited that compared with the ischemia control group,the MVD was significantly greater at 7, 14, 21, 28 days after transplantation in the transplantation treated group (P ＜ 0.05-0.01).Results indicated that neural stem cells differentiated from human adipose tissue-derived stromal cells transplantation can promote the angiogenesis in cerebral ischemia regions.关键词
脂肪基质细胞/神经干细胞/脑缺血/血管新生/微血管密度/干细胞移植分类
医药卫生引用本文复制引用
刘斌,刘宁,董静,王瑞敏,张晋霞..脂肪来源神经干细胞移植局灶性脑缺血模型大鼠的血管新生[J].中国组织工程研究与临床康复,2011,15(1):23-28,6.基金项目
河北省自然科学基金课题(C2008000994),河北省优秀专家出国培训基金课题(2007),唐山市科研基金课题(08130204a-1-21).课题名称为人脂肪神经干细胞培养及移植治疗大鼠局灶性脑缺血损伤的实验研究. （C2008000994）
