中国组织工程研究与临床康复2011，Vol.15Issue(2)：298-301,4.DOI:10.3969/j.issn.1673-8225.2011.02.026
内皮型一氧化氮合酶基因转染对血管损伤后新生内膜增生的影响
Effects of endothelial nitric oxide synthase gene transfection on neointima hyperplasia after vascular injury
摘要
Abstract
BACKGROUND:Nitric oxide (NO) can inhibit the proliferation and migration of vascular smooth muscle cells (VSMCs) and endothelial nitric oxide synthase (eNOS) is an important factor of the synthesis of NO. However, the effects of eNOS gene transfection on neointimal hyperplasia after balloon injury in atherosclerosis rats remain unclear.OBJECTIVE: To study the inhibition effects of eNOS gene transfection on neointimal hyperplasia after balloon injury in atherosclerosis rats.METHODS:The common carotid catheter inju ry model in atherosclerosis rats was established. All animals were divided into blank control, AdCMV-lacz and AdCMV-eNOS groups. Immediately after angioplasty, AdCMV-eNOS or AdCMV-lacZ or PBS was transfected into the injured segment of the common carotid artery for local therapy. Two weeks later, the transfected vascular were obtained to detect expression of eNOS mRNA in cultured smooth muscle cells. And then intimal-media ratio and neointimal hyperplasia were measured at different time points.RESULTS AND CONCLUSION:The transfected common carotid arteries were confirmed the mRNA expression of eNOS gene in cultured smooth muscle cells. At 1 and 3 months after transfection, the intimal-media ratio was significantly reduced in AdCMV-eNOS transfected group than PBS group or AdCMV-lacZ transfected group (P ＜ 0.01). eNOS gene transfection might strongly inhibit neointima hyperplasia after balloon injury in atherosclerosis rats and reduce arterial restenosis.关键词
内皮型一氧化氮合酶/基因转染/血管平滑肌细胞/新生内膜/血管损伤/组织构建分类
医药卫生引用本文复制引用
胡萍,盛净,蔡文玮,陆平..内皮型一氧化氮合酶基因转染对血管损伤后新生内膜增生的影响[J].中国组织工程研究与临床康复,2011,15(2):298-301,4.基金项目
上海高校选拔培养优秀青年教师科研专项基金资助项目(jdy07069),项目名称:Ad-CMVeNOS 基因转染抑制新生内膜增生的实验研究. （jdy07069）
