原子能科学技术2011，Vol.45Issue(1)：124-128,5.
咖啡因联合辐射处理对人乳腺癌易感基因BRCA1表达的影响
Effects of Caffeine Cotreatment With Radiation on Breast Cancer Susceptibility Gene BRCA1
摘要
Abstract
The sensitizing effect of caffeine to carbon ion radiation was investigated and the change of BRCA1 expression was observed.The MCF-7 breast carcinoma cells were exposed to carbon ion beams with or without caffeine.The cell survival was automatically monitored by RT-CES system.Cell cycle distribution was assessed by flow cytometry.The levels of BRCA1 mRNA were analyzed by real-time RT-PCR.The expression of BRCA1 protein and its phosphorylation were examined by Western blot.The results show that caffeine increases the sensitivity of MCF-7 cells to carbon ion radiation, and abrogates the radiation-induced G2 arrest.Caffeine inhibits radiation-induced BRCA1 expression both at mRNA and protein level.At the same time, caffeine specifically abolishes BRCA1 phosphorylation of Ser-1524.The data implicate that caffeine inhibits the expression of BRCA1 protein and its phosphorylation.关键词
BRCA1/离子辐射/咖啡因/辐射敏感性/G2期检测点分类
生物科学引用本文复制引用
李宁,张红,王燕玲,周鑫,郝冀方..咖啡因联合辐射处理对人乳腺癌易感基因BRCA1表达的影响[J].原子能科学技术,2011,45(1):124-128,5.基金项目
国家自然科学基金资助项目(10835011,10805064) （10835011,10805064）
甘肃省中青年科技基金资助项目(099RJYA015 ) （099RJYA015 ）
