中国组织工程研究与临床康复2011，Vol.15Issue(1)：183-186,4.DOI:10.3969/j.issn.1673-8225.2011.01.042
CpG寡脱氧核苷酸对外周血单个核细胞功能的影响:1型糖尿病患者与健康者对照
Effects of CpG oligonucleotides on the function of peripheral blood mononuclear cells in patients with type 1 diabetes mellitus versus healthy volunteers
田德增 1刘明哲 1张永选 1魏晓华 1任保仙 1王海敏1
作者信息
- 1. 河南省濮阳市安阳地区医院内分泌科,河南省安阳市,455000
- 折叠
摘要
Abstract
BACKGROUND: CpG oligonucleotide has been shown to strengthen the function of peripheral blood mononuclear cells (PBMCs), but its effects on type 1 diabetes mellitus has been rarely reported. OBJECTIVE: To investigate the effects of CpG oligonucleotide on the expression of interferon γ (IFN-γ), interleukin (IL) -12 and IL-10 in PBMCs in patients with type 1 diabetes mellitus versus healthy controls. METHODS: PBMCs were isolated from patients with type 1 diabetes mellitus and healthy controls and then cultured in RPMI-1640 with non-stimulator (control group) and CpG oligonucleotide (CpG oligonucleotide group), respectively. The mRNA expression of IFN-γ, IL-10, and IL-12 in PBMCs was detected by reverse transcription-polymerase chain reaction. RESULTS AND CONCLUSION: mRNA expression of IFN-γ and IL-10 was significantly lower in patients with type 1 diametes mellitus than in healthy controls (P < 0.01). In the CpG oligonucleotide group, the mRNA expression of IFN-γand IL-12 was significantly higher than in the healthy control group (P < 0.01), but the mRNA expression of IL-10 was similar to that in the healthy control group (P > 0.05). These findings demonstrated that CpG oligonucleotide can promote the production of IFN-γ and IL-12 in PBMCs of type 1 diametes mellitus.关键词
CpG寡脱氧核苷酸/γ干扰素/白细胞介素/1型糖尿病分类
医药卫生引用本文复制引用
田德增,刘明哲,张永选,魏晓华,任保仙,王海敏..CpG寡脱氧核苷酸对外周血单个核细胞功能的影响:1型糖尿病患者与健康者对照[J].中国组织工程研究与临床康复,2011,15(1):183-186,4.
