中国组织工程研究与临床康复2011，Vol.15Issue(3)：567-570,4.DOI:10.3969/j.issn.1673-8225.2011.03.046
海藻酸钠-多聚赖氨酸-海藻酸钠微囊内细胞浓度与胰岛素和C肽的释放
Release of insulin and C peptide associated with cell concentration in alginate-polylysine- alginate microcapsules
摘要
Abstract
BACKGROUND: Microencapsulation has been widely used in various experimental studies, microencapsulated stem cell therapy for diabetic mellitus has become a hot issue, but the relationship between the wrapped cell concentration and the insulin release becomes one of the problems required to be solved.OBJECTIVE: To investigate the influence to the release of insulin and C peptide by the density of the insulin-producing cells (IPCs) encapsulated by alginate-polylysine-alginate microcapsules.METHODS: The extracts of rat pancreatic inj ury were prepared, and mouse bone marrow mesenchymal stem cells were induced to differentiate into IPCs. Immunofluorescence and dithizone staining were used to identify the expression of insulin in the induced cells. IPCs were prepared into cell suspension at 1×107/L, 5×104/L, 1×108/L, 5×108/L, 1×109/L, 5×109/L, and then prepared microcapsules by gas blowing spray. Cell viability was detected with 6-carboxyl fluorescein diacetate. Glucose was used to stimulate the cells in microcapsules and to examine the release of insulin and C peptide.RESULTS AND CONCLUSION : After extracts of rat pancreatic injury were induced, the immunofluorescence and dithizone staining have identified the insulin expression in IPCs; IPCs microcapsule was approximately 400 um diameter with uniform size,6-carboxyl fluorescein diacetate detected that cell vitality was good. Glucose was used to stimulate the microcapsules at different cell densities, results found that the secretion of insulin and C peptide reach a peak at cell density of 1×108/L..关键词
微囊化/骨髓间充质干细胞/胰岛素产生细胞/胰岛素/C肽分类
医药卫生引用本文复制引用
王雅光,马云胜,穆长征,蔡红玉,王征..海藻酸钠-多聚赖氨酸-海藻酸钠微囊内细胞浓度与胰岛素和C肽的释放[J].中国组织工程研究与临床康复,2011,15(3):567-570,4.基金项目
辽宁省自然科学基金(20072201) （20072201）
辽宁省教育厅创新团队项目(2006T062). （2006T062）
