中国组织工程研究与临床康复2011，Vol.15Issue(5)：783-787,5.DOI:10.3969/j.issn.1673-8225.2011.05.006
乙肝病毒相关肝病患者肝移植后外周血单个核细胞及肝组织中乙型肝炎病毒cccDNA的检测
Detection of hepatitis B virus cccDNA in peripheral blood monuclear cells and liver tissues in hepatitis B virus-related disease patients after liver transplantation
摘要
Abstract
BACKGROUND: Life-Iong prophylaxis against hepatitis B virus (HBV) recurrence is recommended in patients transplanted for HBV-related disease. But exact mechanism of HBV recurrence and its copy law is unknown.OBJECTIVE: By detection of HBV cccDNA and HBV DNA in peripheral blood mononuclear cells (PBMCs) and liver tissues and serum from HBV-related end-stage liver disease patients before and after liver transplantation.METHODS: PBMCs were isolated from HBV-related end-stage liver disease patients by lymphocyte separation medium.HBVcccDNA in PBMCs and liver tissue and the serum HBVDNA at three periods of before, after liver transplantation and postoperative recurrence were detected by fluorescent quantitative PCR.RESULTS AND CONCLUSION: Before liver transplantation, cccDNA in PBMCs and liver tissues were positive in 12 cases and 6 cases, the detection rates were 32.43％ and 16.22％, cccDNA copies in PBMCs ranged from 3.028 to 6.508×104 copies/mL, as in liver tissues the range was from 4.158 to 6.234 × 104 copies/mL. After liver transplantation, cccDNA in PBMCs was positive in one case. In 6 cases of hepatitis B recurrence after liver transplantation, cccDNA in PBMCs was positive in 4 cases, as positive in one case of liver biopsy, and all 6 cases positive for serum HBVDNA. Possible way of hepatitis B recurrence of HBV-related end-stage liver disease patients after liver transplantation may be that residual HBV in peripheral mononuclear cells replicate according to cccDNA as the template, and then migrate to the liver.关键词
乙型肝炎病毒/乙型肝炎病毒cccDNA/单个核细胞/肝移植/乙肝复发分类
医药卫生引用本文复制引用
张荣,吴忠均,曹辰,陈映..乙肝病毒相关肝病患者肝移植后外周血单个核细胞及肝组织中乙型肝炎病毒cccDNA的检测[J].中国组织工程研究与临床康复,2011,15(5):783-787,5.基金项目
国家自然科学基金(30772055)项目 （30772055）
重庆市卫生局科研项目(06-2-017).课题名称:乙肝相关性肝病肝移植术后乙肝复发的机制及干预研究. （06-2-017）
