中国组织工程研究与临床康复2011，Vol.15Issue(5)：778-782,5.DOI:10.3969/j.issn.1673-8225.2011.05.005
大鼠近交系间原位肝移植急性排斥模型的建立与评价
Establishment and evaluation of an acute rejection model of orthotopic liver transplantation in inbred rats
摘要
Abstract
BACKGROUND:The models commonly used from closed population at present were not well ideal, which easy lead to liver transplantation tolerance.OBJECTIVE:To establish a stable rat model of acute rejection following allograft liver transplantation from DA to Lewis by two-cuff method.METHODS:The rats were allocated to the isogene group (n=24, liver grafts from Lewis rat to Lewis rat) or allogene group (n=24, liver grafts from DA rat to Lewis rat). The general states and survival duration of the rats after transplantation were observed. Three recipient rats in each group were randomly executed at 3, 5, 7 and 10 days for observation of histopathological changes in the liver tissues and detection of serum levels of aspartate aminotransferase (AST), total bilirubin,and cytokine.RESULTS AND CONCLUSION:In the isogene group,a mild morphological change in the liver tissue was found but no acute rejection occurred, which the median survival duration was more than 100 days. In the allogene group, however, obvious jaundice developed postoperatively, which the median survival duration was 11 days, and a typical acute rejection occurred pathologically in the liver tissue at 7 days according to the Banff standards. Levels of AST, total bilirubin, and cytokine were respectively significantly higher in allogene group than that in isogene group at 3, 5, 7 and 10 days (P ＜ 0.001). The acute rejection of DA to Lewis orthotopic liver transplantation model is stable; it can be effectively used to study allograft rejection and tolerance.关键词
近交系大鼠/肝移植/排斥反应/免疫耐受/动物模型分类
医药卫生引用本文复制引用
谢金敏,董瑞强,李涛,张会迎,姚坤厚,温浩..大鼠近交系间原位肝移植急性排斥模型的建立与评价[J].中国组织工程研究与临床康复,2011,15(5):778-782,5.基金项目
广东省科技计划项目(37055)"凋亡细胞诱导肝移植免疫耐受的方法及相关机制研究". （37055）
