中国组织工程研究与临床康复2011，Vol.15Issue(5)：861-864,4.DOI:10.3969/j.issn.1673-8225.2011.05.024
雷公藤甲素PG490-88联合环孢素A免疫抑制诱导肾移植急性排斥反应模型大鼠的免疫耐受
Triptolide PG490-88 combined with ciclosporin A induces immune tolerance in a rat renal allograft rejection model
摘要
Abstract
BACKGROUND:Studies demonstrated that triptolide has good effect on anti-rejection and anti-tumor. PG490-88 is extracted from triptolide, which can prevent graft versus host disease and induce immune tolerance.OBJECTIVE:To investigate the effect of PG490-88 combined with ciclosporin A on inducing immune tolerance in a rat renal allograft rejection model.METHODS:Kidney transplantation models were constructed using improved method. Forty Wistar rats served as donors, and 40 SD rats served as recipients. All rats were randomly divided into 4 groups. Rats were regular raised in the control group. Rats were lavaged with triptolide PG490-88 [20 mg/(kg·d)], ciclosporin A [15 mg/(kg·d)] or PG490-88 [20 mg/(kg·d)]+ ciclosporin A [15 mg/(kg·d)] in the PG490-88, ciclosporin A or the combination group. Serum levels of interleukin 2-receptor (IL-2R) and activity of IL-2 in spleen lymph cells were detected respectively at 1, 3, 5, 7 and 14 days after transplantation.RESULTS AND CONCLUSION:Serum levels of IL-2R and activity of IL-2 were detected lower in 3 treated groups than those in the control group (P ＜ 0.05). Among the 3 treated groups, the combination group was lower than that of the PG490-88 and ciclosporin A groups (P ＜ 0.05). Triptolide PG490-88 performs its function of immunosuppressant on the liver transplantation through IL-2 and IL-2R. It is not only has immunosuppressive action, but also can induce immune tolerance, the effects would be better when combined with ciclosporin A.关键词
雷公藤甲素/肾移植/免疫抑制/排斥反应/抗排异分类
医药卫生引用本文复制引用
戴澄,王平,张新涛,黄志恒,朱量..雷公藤甲素PG490-88联合环孢素A免疫抑制诱导肾移植急性排斥反应模型大鼠的免疫耐受[J].中国组织工程研究与临床康复,2011,15(5):861-864,4.基金项目
深圳市科技计划项目基金(200903038) . （200903038）
