新等位基因人类白细胞抗原B*15:133的鉴定及其原核表达OACSCDCSTPCD
Identification of a human new allele human leucocyte antigen-B*15:133 and its prokaryotic expression
背景:国际上至2010-04共报告人类白细胞抗原4 633个等位基因,中国自2000年至少发现200个新的等位基因,由于资金和时间有限,大部分新等位基因未作血清分型、家系研究以及进一步的功能性研究.目的:在健康志愿者人群中发现新等位基因,并对其进行家系研究,同时构建重链胞外区多肽的原核细胞表达载体,鉴定其在原核细胞中是否表达.方法:利用PCR-SSO和PCR-SBT技术,对人群的人类白细胞抗原进行筛选.使用血清学和SBT技术对拥有新等位基因的供者…查看全部>>
BACKGROUND: In total 4 633 alleles have been reported in world up to April 2010. More than 200 new alleles have been found in China, most of which need to be further studied.OBJECTIVE: Serologic and genetic studies were performed to confirm the identity of a new human leucocyte antigen (HLA)allele within the Chinese population to construct an expression vector containing the heavy chain of HLA-B*15:133 in a prokaryotic system and to identify its activity.MET…查看全部>>
王琳;宋长兴;张志欣
北京市结核病胸部肿瘤研究所,北京市,101149北京市红十字血液中心,北京市,100088北京市红十字血液中心,北京市,100088
基础医学
新等位基因人类白细胞抗原BPCR-SSOPCR-SBT原核表达表达载体pET30a(+)
《中国组织工程研究与临床康复》 2011 (5)
878-881,4
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