西安交通大学学报（医学版）2011，Vol.32Issue(2)：246-249,4.
Bmi-1基因过度表达与喉癌的分化、转移及预后的关系
Association of Bmi-1mRNA overexpression with differentiation,metastasis and prognosis of laryngocarcinoma
摘要
Abstract
Objective To investigate the association of B cell-specific MLV integrationsite-1 (Bmi-1) mRNA expression level with the differentiation, metastasis and prognosis of laryngocarcinoma. Methods Tissue specimens were obtained from 52 patients undergoing surgery for laryngocarcinoma between May and November 2008 in our hospital. Reverse transcriptional PCR (RT-PCR) was performed for amplification of Bmi-1 mRNA from the 52 tumor tissues and matched adjacent normal tissues, and the differential Bmi-1 mRNA expression was analyzed for its association with the clinical manifestations of the patients. Results Bmi-1 mRNA and protein expressions were detected in all the laryngocarcinoma tissues and normal tissues adjacent to the carcinoma using fluorescence method, and in 39 cases Bmi-1 mRNA expression was significantly higher in the tumor tissues than in the adjacent tissues. In 32 cases of tumor tissue, Bmi-1 protein expression was significantly higher than the adjacent normal laryngeal tissue. The expression of Bmi-1 mRNA was highly correlated with tumor size, lymph node metastasis and T classification (P＜0.05), but not with the patients' gender, age or tumor differentiation (P＞0.05).Conclusion Brai-1 mRNA expression is closely correlated with the differentiation and metastasis of laryngocarcinoma.Bmi-1 mRNA may serve as a marker for assessing the progression of laryngocarcinoma and providing assistance for clinical therapeutic decisions and may facilitate its prognostic evaluation.关键词
喉癌/Bmi-1/基因表达/肿瘤转移/预后分类
医药卫生引用本文复制引用
姚小宝,王晓侠,张少强,闫利英,朱宏亮..Bmi-1基因过度表达与喉癌的分化、转移及预后的关系[J].西安交通大学学报（医学版）,2011,32(2):246-249,4.基金项目
国家自然科学基金国际合作项目资助(No.30210103151) （No.30210103151）
