中国动脉硬化杂志2011，Vol.19Issue(1)：29-33,5.
PCSK9-siRNA在抗ox-LDL诱导的THP-1源性巨噬细胞凋亡中对Bax、Bcl-2表达的影响
Effects of PCSK9 siRNA on Bax, Bcl-2 Expression in THP-1 Derived Macrophages Apoptosis Induced by ox-LDL
摘要
Abstract
Aim To explore the effect of PCSK9 siRNA on Bax, Bcl-2 expression in THP-1 derived macrophages apoptosis induced by ox-LDL Methods Cells were incubated with varied concentrations of ox-LDL for different time.Western blot were conducted to detect the expression of Bax and Bcl-2. The siRNAs for PCSK9 were designed and synthesized, then THP-1 macrophages were transfected with PCSK9 siRNA (30, 50, 80 nmol/L) for 24 h and then a high level of ox-LDL (80 μg/mL) for an additional 48-hr. Bax , Bcl-2 protein levels were measured by Western blot and apoptosis was measured with Hoechst 33258 staining. Results ox-LDL (20, 40, 60, 80 μg/mL) increased Bax expression in a dose-dependent manner, but inversely decreased Bcl-2 expression. Furthermore, 80 μg/mL ox-LDL increased Bax expression in a time-dependent manner, but inversely decreased Bcl-2 expression. However, PCSK9 siRNA attenuated ox-LDL-induced up-regulation of Bax, down-regulation of Bcl-2, and THP-1 cell apoptosis in a dose-dependent manner.Conclusion The effect of PCSK9-siRNA aganist ox-LDL-induced THP-1 cell apoptosis is associated with down-regulating of Bax and up-regnlating of Bel-2.关键词
枯草溶菌素转换酶9/巨噬细胞/细胞凋亡/Bax/Bcl-2分类
医药卫生引用本文复制引用
唐志晗,武春艳,任重,刘录山,姜志胜..PCSK9-siRNA在抗ox-LDL诱导的THP-1源性巨噬细胞凋亡中对Bax、Bcl-2表达的影响[J].中国动脉硬化杂志,2011,19(1):29-33,5.基金项目
湖南省应用基础研究计划重点项目(2008FJ2006),湖南省科技厅计划项目(2009TP4057-2,2010TP4008-2),湖南省教育厅重点科研项目(10A105)及湖南省高校科技创新团队支持计划资助项目. （2008FJ2006）
