中国临床医学2011，Vol.18Issue(1)：11-14,4.
脂多糖诱导激活人脐静脉内皮细胞 Notch 信号通路
Activation of Notch Signaling System in Primary Human Umbilical Vein Endothelial Cells by Lipopolysaccharide
摘要
Abstract
Objective:To investigate the role of Notch signaling in lipopolysacharide (LPS) induced inflammation response in primary human umbilical vein endothelial cells (HUVEC).Methods: Primary human umbilical vein endothelial cells were cultured with or without lipopolysacharide.HUVEC were pretreated with DMSO vehicle control or γ-secretase inhibitor (DAPT) for 16 hours and subsequently stimulated with LPS.Quantitative real-time PCR were performed to measure the mRNA level of HES1.Results: LPS stimulation induced the expression of the canonical Notch target gene HES1.Peak expression of HES1 mRNA was observed at 0.5 hour after LPS stimulation, and amounts of mRNA increased in a dose-dependant manner.LPS stimulation did not increase the expression of HES1 when cells were pretreated with DAPT.Conclusions: The expression of the canonical Notch target gene HES1 is effectively induced by LPS, suggesting that the activation of Notch signaling pathway is involved in the inflammatory response induced by LPS.关键词
脂多糖/Toll样受体/内皮细胞/炎症/Notch信号途径分类
医药卫生引用本文复制引用
臧敏华,姜萌,沈敏婕,王永亭,杨国源,何奔,张彦周..脂多糖诱导激活人脐静脉内皮细胞 Notch 信号通路[J].中国临床医学,2011,18(1):11-14,4.基金项目
国家自然基金项目(编号:30800453) （编号:30800453）
上海市青年科技启明星计划资助(编号:10QA1404500) （编号:10QA1404500）
上海交通大学"医工(理)交叉研究基金" 项目(编号:YG2010MS29) （理）
