中国临床医学2011，Vol.18Issue(1)：19-21,3.
柯萨奇病毒 B3 诱导 HeLa 细胞凋亡
Coxsackievirus B3-Induced Apoptosis in HeLa Cells
摘要
Abstract
Objective: To observe the dynamic process of apoptosis in HeLa cells induced by Coxsackievirus B3 (CVB3). Methods: After infection with CVB3, HeLa cells were harvested at different time points,cytopathic effect and apoptosis were detected by contrast microscopy, electron microscopy, flow cytometry and terminal deoxynucleotidyl transferase mediated dUTP nickend labeling(TUNEL). Results: Apoptosis of HeLa cells occured in 4 hours after coculture with CVB3, (11.79％ ± 0. 962％vs. 4.07％ ±0. 665％, P＜0. 05),which peaked at 36-48h post-infection. However, apoptosis was inhibited in HeLa cells at 1 hour post-infection with CVB3 (2.21％ ± 0. 448％ vs. 3.73％ ± 0. 637％, P＜0.05). Conclusions: CVB3 infection induces apoptosis in HeLa cells, and virus-associated apoptosis may play an important role in the pathogenesis of cytopathic effect.关键词
柯萨奇病毒B3/HeLa细胞/凋亡分类
医药卫生引用本文复制引用
李小平,陈瑞珍,宿燕岗,陈灏珠,李浪,刘唐威,伍伟锋..柯萨奇病毒 B3 诱导 HeLa 细胞凋亡[J].中国临床医学,2011,18(1):19-21,3.基金项目
上海市自然科学基金(编号07RZ14022) （编号07RZ14022）
