中国康复2011，Vol.26Issue(1)：6-9,4.DOI:10.3870/zgkf.2011.01.002
佐剂关节炎大鼠心功能变化与细胞因子、调节T细胞和Foxp3表达的相关性
Cardiac Function in Rats with Adjuvant Arthritis and Its Relationship with Cytokines, Regulatory T Cells and the Expression of Foxp3
摘要
Abstract
Objective: To observe paw swelling (E) and arthritis index (AI), cardiac function, cytokines, regulatory T cells (Tregs) and the expression of Foxp3 in rats with adjuvant arthritis (AA). Methods: Twenty-four Wistar male rats were randomly divided into normal control (NC) group and AA model group, 12 in each group.The AA model was established by intradermal injection of Freund's complete adjuvant (FCA) in the right paw in the AA model group. One day before, and 18 and 32 days after AA establishment, body weight, E and Al were determined, and at the 48th day, cardiac function, peripheral blood interleukin-6 (II.-6), IL-10, IL-17, tumor necrosis factor alpha (TNF-α), Tregs, the expression of Foxp3 in the spleen were tested. Results: (1) Compared with NC group, HR, cardiac mass index (HI), left vent ricular end systolic pressure (LVSP) were significantly increased,left ventricular pressure ±dp/dtmax was significantly decreased, body weight was significantly decreased, E and Al were significantly increased, serum TNF-α, IL-17 and IL-6 levels were significantly increased, and the IL-10 level was significantly decreased, the expression of CD4 +Tregs, CD25 +Tregs, CD4 +CD25 +Tregs, and that of Foxp3 in the spleen were significantly down-regulated in AA model group; (2) Spearman correlation analysis showed that, cardiac hemodynamic function parameters were significantly correlated with Al, cytokines, Tregs and Foxp3 (P＜ 0.05 or P＜ 0.01 ). Conclusion: The cardiac function, and the expression of Foxp3, CD4 + CD25 +Tregs were reduced spontaneously in the AA rats with increased E and AI, suggesting inflammation, and the imbalance between Foxp3 and CD4+CD25+ Tregs may be the major factors for cardiac function changes in AA rats.关键词
佐剂性关节炎/心功能/细胞因子/Foxp3的表达/调节T细胞分类
医药卫生引用本文复制引用
朱艳,刘健,曹云祥..佐剂关节炎大鼠心功能变化与细胞因子、调节T细胞和Foxp3表达的相关性[J].中国康复,2011,26(1):6-9,4.基金项目
国家中医药重点学科中医痹病学建设项目--国中医药发[2009]30号 （）
安徽省科技厅科研计划项目(No.09020304046) （No.09020304046）
安徽省卫生厅中医药科研项目(No.2009ZY05) （No.2009ZY05）
安徽中医内科应用基础与开发研究省级实验室项目(科条[2008]150号) （科条[2008]150号）
安徽省教育厅自然科学重点科研项目(No.KJ2008A165) （No.KJ2008A165）
安徽中医学院科技创新团队项目(No.2010TD005) （No.2010TD005）
