中国美容医学2011，Vol.20Issue(2)：240-243,4.
6-O-羧甲基壳聚糖对人增生性瘢痕成纤维细胞TGF-β1表达的影响
Effects of 6-O-carboxymethyl-chitosan on the expression of TGF-β1 in human hypertrophic scar fibroblasts
吴丹 1匡瑞霞 2王志国 2陈璐 2周明 1黄洪杰1
作者信息
- 1. 青岛大学医学院,山东,青岛,266021
- 2. 青岛大学医学院附属医院整形美容中心,山东,青岛,266013
- 折叠
摘要
Abstract
Objective To observe the effects of 6-O-carboxymethyl-chitosan (6-O-CMC) with different concentrations on the expression of transforming growth factor-beta1 (TGF-β1) in human hypertrophic scar fibroblasts (HSFBs) in vitro.Methods HSFBs were cultured in vitro,and the 3～5 generation cells were chose to the next experiment.The DMEM medium with different concentrations of 6-O-CMC (100,200,400ug/ml) were used in the experimental groups, and the control group without 6-O-CMC.After 24 hours, cell morphology and growth state of HSFBs were observed under the microscope.The expression of TGF-β1 protein was detected by ELISA.The expression of TGF-β1 mRNA was was detected by Real-time RT-PCR.Results Compared with the control group, the expressions of HSFBs TGF-β1 protein and mRNA in the other groups all reduced obviously (P＜0.05).Concentration and expression were in inverse proportion (P＜0.05).The expression of TGF-β1 protein and mRNA are on the same direction.Conclusions 6-O-CMC could suppress the expression of HSFBs TGF-β1.We exploring the mechanism of 6-O-CMC in treating hypertrophic scar,which provides theoretical basis for the development and utilization of new drugs.关键词
6-0-羧甲基壳聚糖/增生性瘢痕成纤维细胞/TGF-β1/ELISA/荧光定量RT-PCR分类
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吴丹,匡瑞霞,王志国,陈璐,周明,黄洪杰..6-O-羧甲基壳聚糖对人增生性瘢痕成纤维细胞TGF-β1表达的影响[J].中国美容医学,2011,20(2):240-243,4.
