中国比较医学杂志2011，Vol.21Issue(2)：36-43,8.DOI:10.3969/j.issn.1671.7856.2011.02.09
SHIV-XJ02170感染恒河猴后期的传代特点和env基因变异
Characteristics of SHIV-XJ02170 Passaging in Chinese-Origin Rhesus Macaques in Late Infection Stage and Sequence Variation of env Gene
摘要
Abstract
Objective To explore the characteristic changes of SHIV-XJ02170 during serial passages in Chinese rhesus macaques in late infection stage and to identify the sequence variation of env gene. Methods Viral bloods from macaque G0401V infected with SHIV-XJ02170 in late infection stage of 5 years were used for passaging 2 monkeys ( G0401V→G0402V→G0403 V ) without CD8 + T deletion and 1 monkey ( G0401V→G0404V ) with CD8 + T deletion. Flow cytometry, viral load detection and sequence alignment were used to analyze the change features during serial passages.Results SHIV-XJ02170 incubated in G0401V showed stable infectivity during late infection for 5 years and its virulence was enhanced after passaging in vivo, especially after CD8 + T cell deletion by injection of CM-T807. G0402V showed a sharp decline of weight and CD4 +T cell counts in peripheral blood, and died suddenly on day 41 after infection. Monkeys G0403 and G0404 showed a viral load peak on day 14, then maintained low viral load level ( 104 - 105copies/mL) up to now after passaging ( 141 d). Sequence alignment analysis showed significant variation in env region and changes of Nglycosites in gpl20 region. Conclusions The infectivity of SHIV-XJ02170 is significantly enhanced during serial passages in vivo and this study provides a firm basis for SHIV-XJ02170 infectious molecular clone construction.关键词
SHIV-XJ02170/CD8+T细胞体内剔除/传代/env/变异分类
医药卫生引用本文复制引用
陶真,蒋虹,杨贵波,李喆,魏强,丛喆,刘强,李悦,刘浩,王卫,金光,陈霆,姚南..SHIV-XJ02170感染恒河猴后期的传代特点和env基因变异[J].中国比较医学杂志,2011,21(2):36-43,8.基金项目
国家十一五科技重大专项课题 (2009ZX10004-402),中央级公益性科研院所基本科研业务费专项资金(DWS 201009). （2009ZX10004-402）
