中国实验动物学报2011，Vol.19Issue(1)：29-33,后插3,6.DOI:10.3969/j.issn.1005-4847.2011.01.007
黄连素降糖调脂机制的研究
Mechanism of berberine regulating glucose and lipid metabolism studied with RT PCR array
摘要
Abstract
Objective To explore the effects of berberine on the weight, blood glucose, lipid metabolism and serum insulin in KKAy mice and to investigate its possible glucose-lowing and lipid-regulating mechanism. Methods KKAy mice were randomly divided into two groups: berberine group (treated vith 250 mg/kg/d berberine, n = 8) and control group (n = 8). The fasting blood glucose, weight, TC, TG, HDL-c, LDL-c and fasting serum insulin were tested. The OGTT test was done. RT PCR array experiment was done using skeletal muscle of KKAy mice. Results Berberine significantly decreased fasting blood glucose, AUC, fasting serum insulin, HOMA-IR index, TC and TG, compared with those of the control group (P ＜ 0.05 or P ＜ 0. 01 ). RT PGR array analysis showed that berberine up-regulated the expression of GLUT4, MAPK8, MAPK14, PPARα,UCP2 and HNF4α,while the expression of PPARγ, CEBP, PGC and resistin were down-regulated ( P ＜ 0. 05 ). Conclusions Berberine acts a function of regulating the levels of fasting blood glucose and lipid metabolism, and moderating insulin resistance in KKAy mice. The glucose-lowing mechanisms may be correlated with p38-MAPK-GLUT4 pathway. PPARα may be involved in the lipid-regulating mechanism.关键词
黄连素/KKAy小鼠/基因芯片/糖尿病/脂代谢紊乱/糖代谢分类
生物科学引用本文复制引用
张茜,向红丁,王姮,肖新华,王彤,朱席琳,李文慧,刘英,袁涛,孙晓方,孙琦..黄连素降糖调脂机制的研究[J].中国实验动物学报,2011,19(1):29-33,后插3,6.基金项目
北京协和医院基金项目(编号:2006119). （编号:2006119）
