中国实验动物学报2011，Vol.19Issue(1)：1-5,5.DOI:10.3969/j.issn.1005-4847.2011.01.001
种间转移扩增法筛选长爪沙鼠微卫星位点
Screening of novel microsatellite DNA markers in Mongolian gerbils by cross-amplification
摘要
Abstract
Objective To screen new microsatellite loci in Mongolian gerbils to develop genetic markers for genetic analysis. Methods 536 mouse loci were randomly selected from GenBank and amplified in Mongolian gerbils genetic DNA.The positive PCR products were sequenced and confirmed as simple sequence repeat (SSR) loci. Result Of these 536 mouse markers, 313 (58.39％) were discretely amplified from the laboratory gerbils. Of these 313 sequenced markers, 130 were confirmed as SSR loci in the gerbil, in which 105 loci ( 80.77％ ) were classified as pure, and 25 loci ( 19.23％ ) as unpure. The homology orthologous between mouse and gerbil is about 24.3％ ( 130/536), and the access number of loci in GenBank is from GU562694 to GU562823. Conclusions There are comparatively high homology between mouse and gerbil,and cross-amplification by mouse microsatellite primers is an efficient way to identify gerbil SSR loci.关键词
小鼠/长爪沙鼠/微卫星/种间转移扩增分类
生物科学引用本文复制引用
谭元卿,李薇,杜小燕,路静,吴艳花,王超,陈振文..种间转移扩增法筛选长爪沙鼠微卫星位点[J].中国实验动物学报,2011,19(1):1-5,5.基金项目
国家自然科学基金项目(No.30570261) （No.30570261）
国家科技支撑计划课题(No.2009BAI83B02) （No.2009BAI83B02）
北京市教委重点项目(No.KZ200910025002). （No.KZ200910025002）
