针刺研究2011，Vol.36Issue(1)：1-6,39,7.
电针对雌激素调节内分泌功能的作用及机制研究
Influence of Electroacupuncture on Efficacy of Estrogen in Regulating Hypothalamic Reproductive Endocrine Activity in Rats
摘要
Abstract
Objective To observe the effect of electroacupuncture (EA) on the efficacy of estrogen-induced regulation of reproductive endocrine in ovariectomized (OVX) rats.Methods Thirty SD rats were randomly divided into control, model (OVX), EA-Guanyuan(CV 4), Estrogen (E) and EA+ E groups (n = 6 in each group).OVX model was duplicated by removing the bilateral ovaries.EA (5 Hz/20 Hz, 1 -2 mA) was applied to "Guanyuan" (CV 4) for 30 min, once every other day and for 3 sessions.Estrogen at doses of 0.5 mg/kg and 0.25 mg/kg was intraperitonealy given to rats in the E group and EA+ E group, respectively.Serum estrogen and cortisol contents were assayed by enzyme linked immunosorbent assay and the expression of hypothalamic gonadotrophin releasing hormone(GnRH)mRNA, G protein-coupled receptor 54(GPR 54)mRNA, kisspeptin-1 (Kiss-1)mRNA and corticotropin releasing hormone (CRH) mRNA were detected by using real time polymerase chain reaction (RT-PCR).Results Compared with the control group, the expression levels of GnRH mRNA, GPR 54 mRNA and Kiss-1 mRNA in hypothalamus in the model group were increased significantly (P＜0.05), while the expression of hypothalamic CRH mRNA was down-regulated obviously ( P＜0.05), and serum cortisol and estrogen levels were reduced significantly (P＜0.05).In comparison with the model group, the expression levels of hypothalamic GnRH mRNA in the EA, E and EA+ E groups, and those of hypothalamic GPR 54 mRNA and Kiss-1 mRNA in the E and EA+ E groups were down-regulated considerably (P＜0.05); whereas hypothalamic CRH mRNA expression levels in the E and EA+ E groups were up-regulated obviously (P＜0.05) ,and serum cortisol and estrogen contents in the E and EA+ E groups were increased remarkably (P＜0.05).Comparison among the EA and EA+ E groups showed that the effects of EA+E group were significantly superior to those of EA group in down-regulating hypothalalmic GnRH mRNA and GPR 54 mRNA expression, and in up-regulating hypothalamic CRH mRNA expression and serum cortisol and estrogen contents (P＜0.05).No significant differences were found between E and EA+ E groups in down-regulating hypothalamic GnRH mRNA,GPR 54 mRNA and Kiss-1 mRNA expression, and in up-regulating hypothalamic CRH mRNA expression and serum cortisol and estrogen levels (P＞0.05).Conclusion EA-CV 4 is able to potentiate the effects of low-dose of estrogen in down-regulating hypothalamic GnRH mRNA,GPR 54 mRNA and Kiss-1 mRNA expression, and up-regulating hypothalamic CRH mRNA expression and raising serum cortisol and estrogen contents in OVX rats, showing a favorable modulation effect on reproductive endocrine activity.关键词
去卵巢/电针/下丘脑/促性腺激素释放激素/G蛋白藕联受体54/肿瘤转移抑制因子-1/促肾上腺皮质激素释放激素/皮质醇/雌激素分类
医药卫生引用本文复制引用
王少军,谭连红,杨永升,乔丽娜..电针对雌激素调节内分泌功能的作用及机制研究[J].针刺研究,2011,36(1):1-6,39,7.基金项目
国家中医药管理局课题(06-07 JP 54)、国家自然科学基金课题(No.81072856) （06-07 JP 54）
