中国药理学通报2011，Vol.27Issue(3)：320-324,5.
表没食子儿茶素没食子酸酯对H2O2诱导的SH-SY5Y神经细胞损伤的保护作用
The protective effect of EGCG on hydrogen peroxide induced SH-SY5Y cell injury
摘要
Abstract
Aim To investigate the protective effect of EGCG on hydrogen peroxide induced SH-SY5Y cells injury in vitro.Methods The protective effect of EGCG was observed by morphological identification,MTT assay, and LDH activity, while the expressions of bax and bcl-2 were detected by real-time PCR.Results Compared with the group of H202 control, EGCG( 50, 100 , 150 , 200 μ mol· L -1 )could relieve the damage of SH-SY5Y cells and increase the survival of the cells,lower their LDH level, and adjust the expression of bax and bcl-2 mRNA.Conclusion EGCG can protect SH-SY5Y cells from the injury induced by hydrogen peroxide.关键词
表没食子儿茶素没食子酸酯/过氧化氢/人神经母细胞瘤细胞/bax/bcl-2/实时荧光定量PCR分类
医药卫生引用本文复制引用
陈旭,胡园,李青山,郭代红,王东晓,刘屏..表没食子儿茶素没食子酸酯对H2O2诱导的SH-SY5Y神经细胞损伤的保护作用[J].中国药理学通报,2011,27(3):320-324,5.基金项目
国家自然科学基金资助项目(No 30500650) （No 30500650）
科技部重大资助专项(No 2009ZXJ09001-026) （No 2009ZXJ09001-026）
