中国药理学通报2011，Vol.27Issue(3)：334-337,4.
扇贝多肽对UVB照射HaCaT细胞后NO释放及热休克蛋白70表达的影响
Effect of polypeptide from Chlamys farreri on UVB-induced NO release and HSP70 expression in HaCaT cells
摘要
Abstract
Aim To observe dynamic changes of nitric oxide( NO )release and inducible nitric oxide synthase ( iNOS )expression in HaCaT cells after UVB radiation ,and to investigate the effect of Polypeptide from Chlamys farreri( PCF )on UVB-induced NO release and heat shock protein 70( HSP70 )expression.Methods The release of NO was assayed by electron spin resonance ( ESR ).Western blot analysis was used to determine the protein levels of iNOS and HSP70.Results NO release was significantly increased after 20 mJ· cm-2 UVB radiation and there were two peaks at 3 h and 24 h respectively.PCF inhibited the release of NO at each time point.iNOS protein expressed six hours after UVB radiation and increased gradually , then reached to peak 18～ 24 h later.iNOS inhibitor reduced the release of NO at 24 h after UVB radiation, but had no effect at 3 h.PCF enhanced HSP70 expression in a dose-dependent manner.Conclusions UVB exposure to HaCaT cells induced expression of iNOS and then the release of NO increased, but in early stages of exposure, NO can also be generated by non-iNOS pathway.PCF may enhance the expression of HSP70.thereby inhibit iNOS expression and reduce the generation of NO, thus protect HaCaT cells against UVB radiation.关键词
UVB/HaCaT细胞/扇贝多肽/一氧化氮/热休克蛋白70/诱导型一氧化氮合酶分类
医药卫生引用本文复制引用
王春波,李金莲,张正洋,陈雪红,韩彦弢..扇贝多肽对UVB照射HaCaT细胞后NO释放及热休克蛋白70表达的影响[J].中国药理学通报,2011,27(3):334-337,4.基金项目
国家自然科学基金资助项目(No 30471458) （No 30471458）
