中国药理学通报2011，Vol.27Issue(3)：402-405,4.
前列腺素E1预处理对缺血/再灌注心肌PKC及HSP70表达的影响
Effects of prostaglandin E1 on PKC and HSP70 in ischemia/reperfusion preconditioning Myocytes
张艳侠 1刘丹花 2角灿武 1付润芳 3程亮星1
作者信息
- 1. 郑州大学基础医学院药理学教研室,河南,郑州,450001
- 2. 国际眼科杂志社,陕西,西安,710054
- 3. 濮阳市人民医院药剂科,河南,濮阳,457000
- 折叠
摘要
Abstract
Aim To investigate the effect of prostaglandin E1( PGE1 ) on regulating the expression of PKC and HSP70 in ischemia/reperfusion preconditioning myocytes.Methods 40 rats were randomly divided into five groups: normal control group( C group ),ischemia/ reperfusion group( I/ R group ) and PGE, 14 , 42 ,126 μg · L-1 preconditioning group.The isolated myocardial ischemia/reperfusion injury model was established with langendorff method, the pathological slices were prepared by HE staining for the observation of morphopathological changes of rat myocardial cells;Western blot technique was used to detect quantitatively the level of PKC expression in myocardial cells; the immunohistochemical method was used to measure the change of HSP70 expression.Results Compared with the I/R group, the myocardial expression of PKC and HSP70 in PCE1 preconditioning group was significantly increased( P ＜ 0.05 ), the morphological changes of ischemia/reperfusion myocardial fiber were improved in PGE, preconditioning groups.Conclusion PGE, preconditioning can improve the morphological changes of ischemia/reperfusion myocardial fiber in rat, increase the expression of PKC and HSP70.and effectively reduce ischemia/reperfusion injury in isolated rat hearts.关键词
前列腺素E1/心肌缺血/再灌注/Western blot/蛋白激酶C/药理性预适应/热休克蛋白70分类
医药卫生引用本文复制引用
张艳侠,刘丹花,角灿武,付润芳,程亮星..前列腺素E1预处理对缺血/再灌注心肌PKC及HSP70表达的影响[J].中国药理学通报,2011,27(3):402-405,4.
