南方医科大学学报2011，Vol.31Issue(2)：272-276,5.
抗小鼠TLR2胞外段单表位抗体TSP-2对脓毒症小鼠肠粘膜核因子-κB和细胞因子表达的影响
Effect of TSP-2 antibody against a single epitope of mouse Toll-like receptor 2 extracellular domain on nuclear factor-kappa B and cytokine expression in the intestine of septic mice
摘要
Abstract
Objective To observe the effect of the antibody TSP-2 against a single epitope of mouse Toll-like receptor 2 extracellular domain (mTLR2ECD) on the expression of nuclear factor-kappa B (NF-kB) and cytokines in the intestinal tissue of septic mice. Method Male BALB/c mice were randomly divided into 4 groups, namely the sham-operated group, model group, TSP-2 treatment group and rabbit IgG treatment group. Sepsis was induced by cecal ligarion and puncture (CLP), and at 6,12 or 24 h after the operation, the ileal tissues were harvested from the mice for HE staining. NF-kB expression was detected with immunohistochemistry. Tumor necrosis factor-a (TNF- α) and interleukin-6 (IL-6) mRNA expressions were detected with qRT-PCR and their protein expressions by ELISA. Results The NF-kB expression in the intestinal tissue significantly increased in the model group as compared with that in the sham-operated group, and decreased after TSP-2 treatment. The model group also showed significantly increased expression levels of TNF-a and IL-6 mRNA and protein in the intestinal tissue (P＜0.05), which were lowered by TSP-2 (P<0.05) but not by rabbit IgG treatment (P＞0.05). Conclusion The TSP-2 antibody can protect the intestine and delay the development of sepsis by inhibiting NF-kB activation and down-regulating TNF-a and IL-6 expressions in mice.关键词
脓毒症/Toll样受体2/TSP-2/核因子-κB/细胞因子/肿瘤坏死因子-α/白细胞介素-6Key words
sepsis/ Toll-like receptor 2/ TSP-2/ nuclear factor-kappa B/ cytokines/ tumor necrosis factor-a/ interleukin-6分类
医药卫生引用本文复制引用
黄恩平,姚君,杨翠兰,罗深秋..抗小鼠TLR2胞外段单表位抗体TSP-2对脓毒症小鼠肠粘膜核因子-κB和细胞因子表达的影响[J].南方医科大学学报,2011,31(2):272-276,5.基金项目
国家青年自然科学基金(30801032) （30801032）
