南方医科大学学报2011，Vol.31Issue(1)：28-32,5.
碘乙酰胺诱导成纤维细胞水通道蛋白1的表达具有能量依赖性
Iodoacetamide-induced aquaporin 1 expression in fibroblasts is energy-dependent
摘要
Abstract
Objective To investigate the impact of energy metabolism at the cellular level on the expression of the water channel protein aquaporin 1 (AQP1). Methods Balb/c mouse fibroblasts were incubated with iodoacetamide (IA) in vitro, and the changes in AQP1 expression were detected by immunoblotting and immunohistochemistry at 0, 4, and 6 h. Results IA induced the expression of AQP1 at 4 and 6 h accompanied with cell death. Reverse transcription PCR showed an increased expression of AQP1 mRNA in the cells. AQP1 expression was also upregulated by the inhibitor of microtubule and cytochrome C oxidase. Conclusion A pretranslational regulation occurs in IA-induced AQP1 expression in mouse fibroblasts, and the up-regulated AQP1 accumulation is characterized by mitochondria-related energy dependence.关键词
水通道蛋白1/碘乙酰胺/成纤维细胞/线粒体Key words
aquaporin 1/ iodoacetamide/ fibroblasts/ mitochondria分类
医药卫生引用本文复制引用
李尔然,洪欣,刘霞,尚俊依,王波,王昆,王秋月,康健..碘乙酰胺诱导成纤维细胞水通道蛋白1的表达具有能量依赖性[J].南方医科大学学报,2011,31(1):28-32,5.基金项目
国家自然科学基金(30470766) （30470766）
高等学校博士学科点专项科研基金(20040159011) （20040159011）
教育部留学归国启动基金(教外司留[2004]527号) （教外司留[2004]527号）
辽宁省重点实验室资助项目(2009S105) （2009S105）
