南方医科大学学报2011，Vol.31Issue(2)：216-220,5.
ClC-3 siRNA抑制鼻咽癌细胞调节性容积回缩
ClC-3 siRNA inhibits regulatory volume decrease in nasopharyngeal carcinoma cells
摘要
Abstract
To investigate the role of C1C-3 chloride channels in regulatory volume decrease (RVD) of nasopharyngeal carcinoma (NPC) CNE-2Z cells. Methods C1C-3 siRNA was transfected into CNE-2Z cells in the presence of the transfection reagent HiPerFect Reagent1". The transfection efficiency of C1C-3 siRNA was detected by flow cytometry. The expression of C1C-3 protein was detected by Western blotting, and the changes of cell volume in 160 mOsmol/L hypotonic solution were determined by image analysis. Results The transfection efficiency of C1C-3 siRNA was (63.8 ±3.8)% (n=3, P<0.01), and compared with the control group, C1C-3 siRNA transfection resulted in a reduction of C1C-3 expression by (60.9 ± 4.0)% (n=3, PO.01). The hypotonic challege (160 mOsmol/L) caused cell swelling and induced RVD. In the control group, hypotonic solution bath for 35 min resulted in a RVD of (42.6±2.8)% (w=20), which was significantly decreased to (10.5 ±4.8)% (n=16) in C1C-3 siRNA-transfected cells, demonstrating a reduction of RVD capacity by 75.4% (P<0.01). Conclusion The capacity of RVD is significantly reduced in CNE-2Z cells by C1C-3 chloride channel protein knock-down via C1C-3 siRNA transfection, indicating an important role of C1C-3 chloride channels in the RVD of CNE-2Z cells.关键词
ClC-3氯通道/siRNA/鼻咽癌细胞/细胞容积Key words
C1C-3 chloride channels/ small interfering RNA/ nasopharyngeal carcinoma cells/ cell volume分类
医药卫生引用本文复制引用
叶东,张海峰,朱林燕,王立伟,陈丽新..ClC-3 siRNA抑制鼻咽癌细胞调节性容积回缩[J].南方医科大学学报,2011,31(2):216-220,5.基金项目
国家自然科学基金(30771106,30870567,30871267) （30771106,30870567,30871267）
