南方医科大学学报2011，Vol.31Issue(1)：39-43,5.
甲基苯丙胺对PC12细胞的毒性损伤作用
Primary investigation of methamphetamine-induced toxicity in PC12 cells
摘要
Abstract
Objective To investigate the mechanism of methamphetamine (METH)-induced toxicity in PC12 cells. Methods PC12 cells were treated with METH for 24 h at the doses of 0,05,1.0,15,2.0, or 2.5 mmol/L. The morphological changes of the cells were observed under inverted microscope after the treatment. MTT assay and flow cytometry were used to assess the cell viability and apoptotic rates, respectively, and the level of nitric oxide (NO) was measured by enzyme reduction method. Results The PC12 cells exposed to METH were morphologically featured by cell shrinkage, dendrite disruption and disappearance of cell reticular formation. METH exposure caused a dose-dependent reduction in the cell viability (P<0.01), resulting in also increased cell apoptotic rate and significant elevation of NO in the cell culture supernatant (P<0.05). Conclusion METH exposure induces cytotoxicity and injury of differentiated PC12 cells, leading to decreased cell viability and increased cell apoptosis and NO level. Cell apoptosis and excessive NO production are involved in METH-induced cytotoxicity.关键词
甲基苯丙胺/PC12细胞/细胞凋亡/一氧化氮/细胞毒性Key words
methamphetamine/ PC12 cells/ cell apoptosis/ nitric oxide/ cytotoxicity分类
医药卫生引用本文复制引用
李丽增,王慧君,兰江维,岳霞,刘超..甲基苯丙胺对PC12细胞的毒性损伤作用[J].南方医科大学学报,2011,31(1):39-43,5.基金项目
国家自然科学基金(30872918) （30872918）
