南方医科大学学报2011，Vol.31Issue(1)：65-68,4.
人NESG1基因慢病毒载体的构建及其在293FT细胞中的表达
Construction of a lentiviral vector containing human NESG1 gene and its expression in 293FT cells
摘要
Abstract
Objective To construct a lentiviral vector carrying human NESG1-EGFP gene and observe its expression in 293FT cells. Methods The CDS region of NESG1 gene was amplified from a plasmid containing the full-length NESG1 sequence and cloned into the lentiviral vector pGC-FU-EGFP by restriction endonuclease Agel digestion and T4 DNA ligase ligation. After transformation into competent E. Coli cells, the candidate clones were identified by PCR and sequencing. The recombinant plasmid and the two packaging plasmids were co-transfected into human embryonic kidney cell line 293FT cells by lipofectamine 2000 to produce the lentiviral particles, and the viral titer was determined. The 293FT cells were infected by the lentiviral particles obtained and the transfection efficiency was assessed under fluorescent microscope. Western blotting was used to detect the expression of NESG1 protein in the transfected cells. Results The lentiviral vector pGC-FU-NESGl-EGFP for NESG1 gene was constructed successfully. Strong green fluorescence was observed in 293FT cells under fluorescent microscope after co-transfection of the cells with the 3 plasmids of lentiviral vector. The virus in the supernatant reached a titer of 2X107 TU/ml. The transfection efficiency of the collected virus exceeded 90% in 293FT cells with a multiplicity of infection of 1. Western blotting identified the presence of NESG1 expression in the transfected 293FT cells. Conclusion The lentiviral vector for NESG1 has been successfully constructed with a high yield of lentivirus, which facilitate further investigation of the roles of NESG1 gene in the development and progression of nasopharyngeal carcinoma.关键词
NESG1/慢病毒/增强型绿色荧光蛋白/鼻咽癌Key words
NESG1/ lentivirus/ enhanced green fluorescent protein/ nasopharyngeal carcinoma分类
生物科学引用本文复制引用
刘真,甄艳,于晓黎,江庆萍,龙捷,方唯意..人NESG1基因慢病毒载体的构建及其在293FT细胞中的表达[J].南方医科大学学报,2011,31(1):65-68,4.基金项目
国家自然科学基金(30870973) （30870973）
广东省自然科学基金(8451051501000314) （8451051501000314）
广东高校优秀青年创新人才培育项目(LYM08084) （LYM08084）
