南方医科大学学报2011，Vol.31Issue(1)：57-60,4.
中波紫外线致16HBE细胞损伤及其机制
Mechanisms of ultraviolet B irradiation-induced injuries in 16HBE cells
摘要
Abstract
Objective To investigate the biological effects of ultraviolet B (UVB) irradiation on human bronchial epithelial cells (16HBE cells) and explore the possible mechanism. Methods The survival rates of 16HBE cells were detected by MTT assay at 12 h after UVB irradiation at different doses (0,10, 30, 50, 70, and 100 J/m2) or at 50 J/m2 for different durations (2, 4, 8,12, and 24 h). The DNA ladder was detected by agarose gel electrophoresis, the cell cycle changes were analyzed by flow cytometry, and the expression of nuclear factor-kB (NF-KB)/p65 protein was assayed by Western blotting following the exposures. Results UVB irradiation of the cells resulted in lowered cell survival rates, DNA fragmentation, S phase arrest and up-regulation of NF-icB/p65 protein expression. Conclusions UVB irradiation can induce growth inhibition and apoptosis of 16HBE cells, in which process NF-kB protein may play a key role.关键词
UVB/16HBE细胞/细胞存活率/NF-kB/细胞凋亡Key words
ultraviolet B/ 16HBE cells/ cell viability/ nuclear factor-kB/ apoptosis分类
医药卫生引用本文复制引用
吕超,丁振华,周美娟..中波紫外线致16HBE细胞损伤及其机制[J].南方医科大学学报,2011,31(1):57-60,4.基金项目
国家自然科学基金(30970673) （30970673）
