中国普通外科杂志2011，Vol.20Issue(2)：129-132,4.
LY294002对胆管癌细胞凋亡的影响
Effect of LY294002 on cell apoptosis of cholangiocarcinoma cell line
尹强 1周海燕2
作者信息
- 1. 中南大学湘雅二医院,普通外科,湖南,长沙,410011
- 2. 中南大学湘雅医学院,病理学系,湖南,长沙,410007
- 折叠
摘要
Abstract
Objective To study the effect of LY294002, an inhibitor of PI3-Kinase, on human cholangiocarcinoma QBC939 cells. Methods The cell proliferation was detected by MTr assays. Apoptosis was analyzed by flow cytometry. The phosphorylation of Akt and expression of caspase9 were assessed by Western-blotting.Results LY294002 inhibited the proliferation and induced apoptosis in human cholangioearcinoma QBC939 cells in a dose-dependent manner. Furthermore, Western-blotting showed that LY294002 attenuated the phosphorylatioin of Akt and up-regulated the expression of caspase3, 9. Conclusions LY294002 up-regulates the expression of caspase3, 9 by inhibition of the activity of PI3-K/Akt signaling pathway, induction of apoptosis and decreasing cell proliferation.关键词
胆管肿瘤/LY294002/细胞凋亡分类
医药卫生引用本文复制引用
尹强,周海燕..LY294002对胆管癌细胞凋亡的影响[J].中国普通外科杂志,2011,20(2):129-132,4.
