中国普通外科杂志2011，Vol.20Issue(2)：159-162,4.
反应停对胆囊癌细胞影响的蛋白质组学研究
Proteomics research of gallblader cancer cell line treatment with thalidomide
朱国民 1钟德玝
2李学明 1刘细平2
作者信息
- 1. 南昌大学第一附属医院,普外一科,江西,南昌,330006
- 2. 中南大学湘雅二医院,普通外科,湖南,长沙,410011
- 折叠
摘要
Abstract
Objective To study the relevant differential expression proteins of GBC-SD cell line after treatment with thalidomide and the molecular mechanism. Methods Proteomic techniques and methods were used to separate the differential expression proteins in GBC-SD cells after treated wiht that idomide ( experiment group ) and control group. Results The differential expression proteins in experiment group and control group, whose spots variance was over 2-fold, were analysed with PDQuest software, and found out 36 protein spots at difference level of expression ( P ＜ 0. 05 ) , including 1 3 spots decreasing and 23 spots increasing in experiment group. Then, 10 spots with more than 3-fold difference of expression level were selected for anslysis, and 7 protein spots were identified. Conclusions Thalidomide has some effect on GBC-SD cells. Study on the function of differential protein can be as a basis for research on the mechanism of thalidomide treatment of gallbladder cancer.关键词
胆囊肿瘤/反应停/蛋白质组学分类
医药卫生引用本文复制引用
朱国民,钟德玝
,李学明,刘细平..反应停对胆囊癌细胞影响的蛋白质组学研究[J].中国普通外科杂志,2011,20(2):159-162,4.
