首页|期刊导航|中国海洋湖沼学报（英文版）|Submesoscale characteristics and transcription of a fatty acid elongase gene from a freshwater green microalgae, Myrmecia incisa Reisigl
中国海洋湖沼学报（英文版）2011，Vol.29Issue(1)：87-95,9.DOI:10.1007/s00343-011-9959-z
Submesoscale characteristics and transcription of a fatty acid elongase gene from a freshwater green microalgae, Myrmecia incisa Reisigl
Submesoscale characteristics and transcription of a fatty acid elongase gene from a freshwater green microalgae, Myrmecia incisa Reisigl
摘要
Abstract
Myrmecia incisa is a green coccoid freshwater microalgae, which is rich in arachidonic acid (ArA, C20: 4ω-6,△,5,8, 11,14), a long chain polyunsaturated fatty acid (PUFA), especially under nitrogen starvation stress. A cDNA library of M. incisa was constructed with λ phage vectors and a 545 nt expressed sequence tag (EST) was screened from this library as a putative elongase gene due to its 56% and 49% identity to Marchantia polymorpha L. and Ostreococcus tauri Courties et Chretiennot-Dinet, respectively. Based upon this EST sequence, an elongase gene designated MiFAE was isolated from M. incisa via 5'/3' rapid amplification of cDNA ends (RACE). The cDNA sequence was 1 331 bp long and included a 33 bp 5'-untranslated region (UTR) and a 431 bp 3'-UTR with a typical poly-A tail. The 867 bp ORF encoded a predicted protein of 288 amino acids. This protein was characterized by a conserved histidine-rich box and a MYxYY motif that was present in other members of the elongase family. The genomic DNA sequence of MiFAE was found to be interrupted by three introns with splicing sites of Introns I (81 bp), II (81 bp), and III (67 bp) that conformed to the GT-AG rule. Quantitative real-time PCR showed that the transcription level of MiFAE in this microalga under nitrogen starvation was higher than that under normal condition. Prior to the ArA content accumulation, the transcription of MiFAE was enhanced, suggesting that it was possibly responsible for the ArA accumulation in this microalga cultured under nitrogen starvation conditions.关键词
Myrmecia incisa/fatty acid elongase/transcription/nitrogen starvation/arachidonic acid (ArA)Key words
Myrmecia incisa/fatty acid elongase/transcription/nitrogen starvation/arachidonic acid (ArA)引用本文复制引用
YU Shuiyan,LIU Shicheng ,LI Chunyang,ZHOU Zhigang..Submesoscale characteristics and transcription of a fatty acid elongase gene from a freshwater green microalgae, Myrmecia incisa Reisigl[J].中国海洋湖沼学报（英文版）,2011,29(1):87-95,9.基金项目
Supported by the National Natural Science Foundation of China (No. 30972243), the Creative Project of the Shanghai Municipal Education Committee (No. 09ZZ167), the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry, the Key Discipline Project of the Shanghai Municipal Education Committee (No.J50701),and the Excellent Thesis Grant from Shanghai Ocean University （No. 30972243）
