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几种常见桔园螨类Wolbachia基因检测与序列分析

邹志文 陈芬 余丽萍 夏斌

生态学杂志2011,Vol.30Issue(3):547-551,5.
生态学杂志2011,Vol.30Issue(3):547-551,5.

几种常见桔园螨类Wolbachia基因检测与序列分析

Gene identification and sequence analysis of Wolbachia in several common mites in citrus orchards

邹志文 1陈芬 1余丽萍 1夏斌1

作者信息

  • 1. 南昌大学生命科学与食品工程学院,南昌330031
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摘要

Abstract

Polymerase chain reaction ( PCR) was adopted to examine the wsp, ftsZ, and 16S rDNA of Wolbachia in Amblyseius orientalis, A. eharai, Euseius nicholsi, Neo.seiulus barkeri, Panonychus citri, and Tetranychus harti from the citrus orchards in Nanchang, Jiujiang, and Ganzhou of Jiangxi Province. It was found that only T. harti from the citrus orchards in Nanchang was infected by Wolbachia, with the fragments length of Wolbachia wsp ( Accession No. GQ162482) , ftsZ ( GQ162483) , and 16S rDNA ( GQ162484) being 593, 758, and 896 bp, respectively, while the other five species were uninfected. The Wolbachia in T. harti was very similar to the group Epo in subgroup B, based on the phylogenetic analysis of wsp gene.

关键词

桔园螨类/Wolbachia/基因检测/序列分析

分类

农业科技

引用本文复制引用

邹志文,陈芬,余丽萍,夏斌..几种常见桔园螨类Wolbachia基因检测与序列分析[J].生态学杂志,2011,30(3):547-551,5.

基金项目

江西省自然科学基金项目(0630147)资助. (0630147)

生态学杂志

OA北大核心CSCDCSTPCD

1000-4890

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