首页|期刊导航|中国海洋湖沼学报（英文版）|Cloning and expression analysis of a ubiquitin gene (UbL40) in the haemocytes of Crassostrea hongkongensis under bacterial challenge

Cloning and expression analysis of a ubiquitin gene (UbL40) in the haemocytes of Crassostrea hongkongensis under bacterial challenge

FU Dingkun ZHANG Yang YU Ziniu

中国海洋湖沼学报（英文版）2011，Vol.29Issue(1)：80-86,7.

中国海洋湖沼学报（英文版）2011，Vol.29Issue(1)：80-86,7.DOI:10.1007/s00343-011-9090-1

Cloning and expression analysis of a ubiquitin gene (UbL40) in the haemocytes of Crassostrea hongkongensis under bacterial challenge

FU Dingkun ¹ZHANG Yang ²YU Ziniu¹

作者信息

1. Key Laboratory of Marine Bio-resources Sustainable Utilization, Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
2. Graduate University of Chinese Academy of Sciences, Beijin
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摘要

Abstract

Ubiquitin, a highly conserved stress-related protein, is assigned multiple functions, such as DNA processing, protein degradation, and ribosome synthesis. The Crassostrea hongkongensis ubiquitin gene (designated ChUbL40) was cloned by a combination of suppressive subtractive hybridization (SSH) and rapid amplification of cDNA ends (RACE). The full-length cDNA of ChUbL40 is 496 bp in length, consisting of a 5' untranslated region (UTR) of 34 bp, a 3'-UTR of 75 bp and an open reading frame of 387 bp encoding a ubiquitin fusion protein of 128 amino acids. Analysis of the amino acid sequence of ChUbL40 reveals that UbL40 is highly conservative during evolution. The expression patterns of ChUbL40 gene in various tissues were examined by real-time PCR. The expression level of ChUbL40 in haemocytes is down-regulated at 4 h and gradually returned to its original level from 6 h to 24 h after Vibrio alginolyticus challenge. Our results suggest that ChUbL40 is ubiquitously expressed and plays an important role in immune defense against bacterial challenge.

关键词

ubiquitin/expression/bacterial challenge/Crassostrea hongkongensis

Key words

ubiquitin/expression/bacterial challenge/Crassostrea hongkongensis

引用本文复制引用

FU Dingkun,ZHANG Yang,YU Ziniu..Cloning and expression analysis of a ubiquitin gene (UbL40) in the haemocytes of Crassostrea hongkongensis under bacterial challenge[J].中国海洋湖沼学报（英文版）,2011,29(1):80-86,7.

基金项目

Supported by the National Basic Research Program of China (973Program) (No. 2010CB126404), the CAS/SAFEA International Partnership Program for Creative Research Teams (No.KZCX2-YW-T001), and the Research Program for Young Scientists at SCSIO （973Program）

中国海洋湖沼学报（英文版）

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ISSN：2096-5508

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