中国实用妇科与产科杂志2011，Vol.27Issue(3)：192-194,3.
子痫前期孕妇外周血中游离胎儿DNA含量变化
Detection of the cell-free fetal DNA in preeclampsia maternal plasma
邬晋芳 1滕菲 2闫桂花2
作者信息
- 1. 西安交通大学医学院第二附属医院妇产科,陕西,西安,710004
- 2. 兰州大学第一医院妇产科,甘肃,兰州,730000
- 折叠
摘要
Abstract
Objective to investigate the content of cell-free fetal DNA in maternal plasma in different gestational age of normal pregnancy and preeclampsia by detecting the u-maspin gene as a fetal DNA marker. Methods 90 cases including 10 cases of nonpregnant healthy, 40 cases of normal pregnancy, 40 cases of preeclampsia were detected content of umaspin gene by using real-time quantitative methylation-specific PCR with relative quantitative assay. Results ( 1 ) 10 healthy nonpregnant samples were negative; (2) 34 cases of normal pregnancy samples were positive, and the difference among three trimesters was significant (P ＜ 0. 05 ). Concentration of second-trimester was 4. 43 times of first-trimester,and concentration of third-trimester was 5. 13 times of first-trimester; ( 3 ) 40 cases of preeclampsia were positive, concentration of mild preeclampsia was 2. 39 times of the normal third-trimester, and concentration of severe preeclampsia was 5.78 times of the normal third-trimester. Conclusion Contect of cell-free fetal DNA in maternal plasma increase as pregnancy advances, and can reflect the severity of the preeclampsia.关键词
u-maspin基因/孕妇外周血/游离胎儿DNA分类
医药卫生引用本文复制引用
邬晋芳,滕菲,闫桂花..子痫前期孕妇外周血中游离胎儿DNA含量变化[J].中国实用妇科与产科杂志,2011,27(3):192-194,3.
