激光生物学报2011，Vol.20Issue(1)：45-49,37,6.DOI:10.3969/j.issn.1007-7146.2011.01.009
转基因水稻表达的Bt蛋白对拟环纹豹蛛(Pardosa pseudoannulata)生长发育的影响
Effects of Bt Protein Expressed by Transgenic Rice on the Development of Wolf Spider( Pardosa pseudoannulata )
摘要
Abstract
In order to appraise Bt protein expressed by transgenic rice transfer and affect the third trophic level creature on the food chain, The natural enemy wolf spider (Pardosa pseudoannulata), which dominates paddy field, was used in this experiment(28±0.5 ℃, R.H = 80 ％, L: D = 12h:12h).Meanwhile, Bt content of the larvae of Rice case worm ( Cnaphalocrocis medinalis) and woff spider in different treating time was quantified.The results showed that a certain amount of Bt protein was measured from Rice case worm treated for 12 h、24 h 、48 h、60 h and spiders for 2 d、4 d 、6 d、8 d、 10 d,respectively,and the Bt content of spider was more than that of Rice case worm, but control group had no Bt trace; significantly higher development and lower mature spiders weight were observed in the wolf spider fed on Bt rice compared with control treatment(p ＜ 0.O1 ); longer spiders life-span than that of management group(p ＜ 0.05 ) though the spiders molting has no significant difference (p ＞ 0.05 ).These results demonstrated the Bt protein expressed by transgenic rice transfer to the third trophic level on food chain, while these protein affect wolf spiders development in a certain extent.关键词
转基因水稻/Bt蛋白/发育历期/食物链/生物富集作用Key words
transgenic rice/ Bt protein/ development time/ food chain/ bioaccumulation分类
生物科学引用本文复制引用
刘立军,黄毅,段妍慧,张志罡,颜亨梅,付秀芹..转基因水稻表达的Bt蛋白对拟环纹豹蛛(Pardosa pseudoannulata)生长发育的影响[J].激光生物学报,2011,20(1):45-49,37,6.基金项目
国家自然科学基金资助项目(30970421 （）
31071878) （）
湖南省科技厅项目(2008SK3066) （2008SK3066）
