激光生物学报2011，Vol.20Issue(1)：103-107,5.DOI:10.3969/j.issn.1007-7146.2011.01.020
拟南芥赤霉素受体AtGID1a原核表达条件的优化研究
Optimization for Prokaryotic Expression Conditions of Gibberellins Receptor AtGID1 a in Arabidopsis
摘要
Abstract
The factors that influenced expression level of Rossetta( DE3 )/pGEX-KG/AtGIDla were optimized using uniform design. The optimization induction condition for the solubility protein expression were investigated. The results were as followed: 16 g/L peptone, 5 g/L yeast powder, 4 g/L glucose, 0. 1 mmol/L IPTG, 23 ℃ induction tempera ture and 4 h induction time. The average density of interest protein had increased from 0.042 to 0. 064. The optimization rate was 52 ％.关键词
pGEX-AtGID1a/均匀设计/偏最小二乘回归/优化表达Key words
pGEX-AtGID1 a/ uniform design/ partial least-squares regressive/ optimization expression分类
生物科学引用本文复制引用
周敏,赵卓亚,徐文忠,肖浪涛,王若仲..拟南芥赤霉素受体AtGID1a原核表达条件的优化研究[J].激光生物学报,2011,20(1):103-107,5.基金项目
湖南省自然科学基金项目(09JJ1004) （09JJ1004）
教育部新世纪优秀人才支持计划项目(NCET-10-0143) （NCET-10-0143）
湖南省科研条件创新专项(2010TY1004) （2010TY1004）
