激光生物学报2011，Vol.20Issue(1)：67-73,7.DOI:10.3969/j.issn.1007-7146.2011.01.013
孝顺竹肉桂醇脱氢酶基因的克隆及表达研究
A Study on the Cloning and Expression of a Cinnamoyl Alcohol Dehydrogenas Gene in Bambusa multiplex (Lour.)Raeuschel
摘要
Abstract
Cinnamoyl Alcohol Dehydrogenas is a key enzyme in the biosynthesis of lignin. A gene of CAD gene family was cloned by RT-PCR and SMART RACE RT-PCR from the shoots of Bambusa multiplex (Lour. ) Raeuschel ( GenBank accession number: GU985522). The cloned CAD gene of Bambusa multiplex ( Lour. ) Raeuschel, with the cDNA length of 1 131 bp, contained an open reading frame of 1 107 bp which encoded a polypeptide of 368 amino acids with predicted molecular mass of 39 kD. The results of sequence analysis showed that the deduced polypeptide contained alcohol dehydrogenase GroES-like domain and zinc-binding dehydrogenase in the conserved sequences of the CAD family. It was highly homologous to the CAD proteins from O. sativa and Zea mays and its identity to CAD proteins from O. sativa was up to 97.0 ％. The phylogenetic tree analysis also indicated that cloned CAD gene has most close relationship with the CAD gene in O. sativa. Tissue specific expression showed that CAD expressed in leaf, sheath, young stem and bamboo shoots, much higher in bamboo shoots. All the study will provide a theoretical basis for further studying molecular regulation mechanism of the CAD gene and for taking advantage of its function by gene engineering technique.关键词
孝顺竹/肉桂醇脱氢酶/克隆/系统进化树分析Key words
Bambusa multiplex ( Lour. ) raeuschel/ cinnamoyl alcohol dehydrogenas/ clone/ phylogenetictree analysis/分类
生物科学引用本文复制引用
卞林玲,范军,程备久,项艳,高健..孝顺竹肉桂醇脱氢酶基因的克隆及表达研究[J].激光生物学报,2011,20(1):67-73,7.基金项目
国家863项目(2006AA100109) （2006AA100109）
安徽省高等学校学科带头人基金项目(2005008)资助 （2005008）
