北京中医药大学学报2011，Vol.34Issue(2)：126-130,5.
微波辅助胶束萃取-高效液相色谱法同时测定平消片中马钱子碱、士的宁和柚皮苷的含量
Quantitative determination of brucine, strychnine and naringin in Pingxiao Tablets simultaneously by microwave-assisted micelle extraction-HPLC*
摘要
Abstract
Objective To establish a simple and rapid microwave-assisted micelle extraction method for separate and detrmine the content of brucine, strychnine and naringin in Pingxiao Tablets. Methods The procedure was performed for 2 minutes under the conditions of 7％ Brij35 as extractive solvent,300W micro power and ratio of liquid to solid as 100:1 (mL/g). The HPLC procedure was performed in gradient elution and the mobile phase was acetonitrile -0.4％ phosphoric acid solution (pH value was adjusted to 3.0 by triethylamine). The detection wavelength was 260 nm. Results Brucine, strychnine and naringin showed a good linear relationship, respectively, at range of 0.630 - 40.7 mg/L, 0.640 - 41.3 mg/L and 9.06 -580 mg/L (r≥0.9993). The average recovery was from 99.7％ to 101.1％ and RSD was from 0.7％ to 1.0％ (n = 5). Conclusion The method established in the test can complete the extraction of the three index constituents within 2 minutes and shorten the pretreatment time, which is simple and rapid with friend environment and accurate and reliable results. It provides a new and dependable approach for quality control and clinical test of Pingxiao Tablets.关键词
微波辅助胶束萃取/高效液相色谱法/平消片/马钱子碱/士的宁/柚皮苷Key words
microwave-assisted micelle extraction/ HPLC/ Pingxiao Tablets/ brucine/ strychnine/ naringin分类
医药卫生引用本文复制引用
卢彦芳,安静,孙婷,蒋晔..微波辅助胶束萃取-高效液相色谱法同时测定平消片中马钱子碱、士的宁和柚皮苷的含量[J].北京中医药大学学报,2011,34(2):126-130,5.基金项目
河北省科学技术研究与发展指导计划资助项目(No.062761956) （No.062761956）
