实用临床医药杂志2011，Vol.15Issue(3)：6-10,5.
不同麻醉下电针对全脑缺血再灌注大鼠脑MDA、SOD及PPAR-γmRNA表达的影响
Effects of electroacupuncture on levels of SOD and MDA in the brain of rats with cerebral ischemia reperfusion injury under different anesthesia
田伟千 1崔苏扬 2姚凤珍 2李宝贵2
作者信息
- 1. 南京中医药大学,江苏南京,210029
- 2. 南京中医药大学附属医院麻醉科,江苏南京,210029
- 折叠
摘要
Abstract
Objective To observe the changes of superoxide dismutase (SOD), malonyldialdehyde(MDA) and PPAR - γmRNA expression in the brain tissues of rats with cerebral ischemia- reperfusion injury and investigate the effects of electroacupuncture on the levels of SOD,MDA and PPAR - γmRNA expression under different anesthesia. Methods Forty male SD rats were uniformly randomized into A, B, C, D and E groups with eight rats in each group. Transient general brain ischemia was induced by four - vessel occlusion and reperfusion under intravenous chloral hydrate or propofol anesthesia. EA (30 to 50 Hz, 1 mA) was applied to Baihui(GV20), Mingmen(GV4) and Zusanli(ST36) for 20 min after IR. The content of SOD and MDA and PPAR -γmRNA expression in the brain tissues were measured postischemia after 24 h. Results SOD levels in group A was significantly lower, while MDA levels and PPAR - γmRNA expression was significantly higher than E group (P＜0.05, P＜0.01, respectively); SOD levels in B and D group were found to have increased, while MDA levels and PPAR - γmRNA expression in groups C and D were found to have decreased in comparison with group E (P＜0.05, P ＜0.01, respectively);SOD levels in group D were found to have increased in comparison with group C ( P ＜ 0.05 ) ;MDA levels and PPAR - γmRNA expression in groups C and D were found to have decreased in comparison with group B (P ＜0.01). MDA was positive correlation with the expression of PPAR- γmRNA . And the correlation coefficient was 0. 664 ( P ＜ 0.01 ). Conclusions Electroacupuncture combined with propofol can protect the brain of cerebral ischemia- repeffusion rat by electroacupuncture improving the activity of SOD and propofol decreasing MDA content and PPAR- γmRNA expression in the brain, and there was an inherent dependability between the MDA and PPAR - γmRNA expression.关键词
脑缺血/再灌注损伤/电针/脑组织丙二醛/超氧化物歧化酶/PPAR-γ/大鼠分类
医药卫生引用本文复制引用
田伟千,崔苏扬,姚凤珍,李宝贵..不同麻醉下电针对全脑缺血再灌注大鼠脑MDA、SOD及PPAR-γmRNA表达的影响[J].实用临床医药杂志,2011,15(3):6-10,5.
