动物营养学报2011，Vol.23Issue(1)：61-65,5.DOI:10.3969/j.issn.1006-267x.2011.01.010
过瘤胃保护性亮氨酸对绵羊骨骼肌哺乳动物雷帕霉素靶蛋白(mTOR)信号传导通路关键因子的影响
Effects of Rumen-protected Leucine on the Key Factors Controlling mTOR Signal Transduction Pathway in the Skeletal Muscle of Sheep
摘要
Abstract
The purpose of this experiment was to study the effects of rumen-protected leucine on the phosphorylation of two key factors (4E-BP1 and p70S6K) of mammalian target of rapamycin ( mTOR) signal transduction pathway in the skeletal muscle of sheep. Twelve sheep were randomly allotted to 4 groups ( with 3 replicates per group and 1 sheep per replicate) , and fed basal diet supplemented with 0 ( control group) , 0. 5, 1. 0 and 1. 5 g/d rumen-protected leucine, respectively. After 15 days, the sheep were slaughtered and the 4E-BP1 and p70S6K phosphorylation degrees in longissimus muscle were determined by Western blot technique. The results showed as follows : compared with the control group , gray values of 4E-BP1 and p70S6K phosphorylation in rumen protected leucine groups were increased ( P ＜0. 05 ), and the highest values were all found in 1. 0 g/d group which were 0. 40％ and 0. 85％ , respectively. Therefore. dietary rumen-protected leucine supplementation can improve the phosphorylation of two key factors ( 4E-BP1 and p70S6K) in mTOR signal transduction pathway in the skeletal muscle of sheep , and the suitable level is 1. 0 g/d. [ Chinese Journal of Animal Nutrition ,2011 ,23 ( 1 ) :61-65 ]关键词
过瘤胃保护性亮氨酸/绵羊/骨骼肌/mTOR分类
农业科技引用本文复制引用
桑丹,孙海洲,郭俊清,赵存发..过瘤胃保护性亮氨酸对绵羊骨骼肌哺乳动物雷帕霉素靶蛋白(mTOR)信号传导通路关键因子的影响[J].动物营养学报,2011,23(1):61-65,5.基金项目
国家自然科学基金(30560106) （30560106）
内蒙古科技创新基金及现代农业技术体系--国家绒毛用羊产业技术体系 （）
