动物营养学报2011，Vol.23Issue(1)：34-42,9.DOI:10.3969/j.issn.1006-267x.2011.01.007
饲粮添加25-羟基维生素D3对轮状病毒攻毒和未攻毒断奶仔猪血清和肠内容物抗体和细胞因子水平的影响
Effects of Dietary Supplementation with 25-OH-D3 on Antibody and Cytokine Levels in Serum and Intestinal Contents of Weanling Pigs with or without Rotavirus Challenge
摘要
Abstract
This experiment was conducted to study the effects of dietary supplementation with 25-OH-D3 on the antibody and cytokines in serum and intestinal contents of weanling pigs with or without rotavirus challenge.Forty-eight 28-day-old three-way cross ( Duroc×Landrace × Yorkshine) weanling pigs with an average body weight of 7. 35 kg were randomly assigned into 4 treatments with 12 replicates and 1 pig in each replicate. The experimental period lasted for 21 d. The pigs of control groups with or without rotavirus challenge (C+ or C- ) and experimental groups with or without rotavirus challenge ( T+ or T- ) were fed diets with 220 and 2200 IU/kg 25-OH-D3 , respectively. The C+ and T+ groups were challenged orally with 1 mL human rotavirus ( 1 × 106 TCID50/mL) . The results showed that dietary supplementation with 2200 IU/kg 25-OH-D3 had elevated the level of rotavirus antibody in serum and intestinal contents of pigs with rotavirus challenge at d 5 and 21 ( P ＜0. 05) . had decreased the serum immunoglobulin G ( IgG) , immunoglobulin M ( IgM) and immunoglobulin A (lgA) contents of pigs without rotavirus challenge at d 5 (P ＜0. 05) . and had reduced the serum IgA content at d 5 and 15 ( P ＜0. 05) and serum IgM content at d 15 ( P ＜0. 01) of pigs with rotavirus challenge. Dietary supplementation with 2200 IU/kg 25-OH-D3 had a decreasing trend of interleukin-2 ( IL-2 )and interleukin-6 ( IL-6 ) levels in serum and intestinal contents and interferon-γ ( IFN-γ) level in intestinal contents of pigs with or without rotavirus challenge ( P ＞ 0. 05 ) . had enhanced the interleukin-4 ( IL-4 ) level in serum and intestinal contents of pigs without rotavirus challenge ( P ＞0. 05 ) . and had increased the serum IL-4 level at d 5 (P＜0. 01) and IL-4 level in intestinal contents at d 15 (P ＜0. 01) of pigs with rotavirus challenge. These results indicate that dietary supplementation with 2200 IU/kg 25-OH-D3 can increase the rotavirus antibody level in serum and intestinal contents with or without rotavirus challenge, reduce the secretion of proinflammatory cytokines and the inflammatory responses concerned with them, promote the production of anti-inflammatory cytokines and the anti-inflammatory responses concerned with them. and enhance the ability of disease resistance of weanling pigs. [ Chinese Journal of Animal Nutrition ,2011 ,23 ( 1 ) :34-42 ]关键词
断奶仔猪/25-OH-D3/轮状病毒/抗体/细胞因子分类
农业科技引用本文复制引用
廖波,张克英,丁雪梅,徐志文,白世平..饲粮添加25-羟基维生素D3对轮状病毒攻毒和未攻毒断奶仔猪血清和肠内容物抗体和细胞因子水平的影响[J].动物营养学报,2011,23(1):34-42,9.基金项目
教育部长江学者和创新团队发展计划资助项目(IRT0555-5) （IRT0555-5）
