实用临床医药杂志2011，Vol.15Issue(3)：11-14,4.
银杏总黄酮对肾缺血再灌注大鼠p-JNK表达的影响
Effect of total flavone of Gingko on phosphorylation of JNK in rats with renal ischemia reperfusion injury
摘要
Abstract
Objective To investigate the change of phosphorylation of JNK in renal ischemia reperfusion injury( IRI)and study the effect of total flavone of Gingko(TFG)on JNK activation. Methods Male Sprague Dawley rats were randomly divided into sham- operation group (Sham, n = 12), ischemia reperfusion groups( IR, n = 30) and TFG groups(TFG, n = 12). The models were established through clamping bilateral renal arteries for 45 rain and then reperfusion.Tubular cell apoptosis was confirmed by terminal deoxynucleotidyl transferase (TDT) mediated dUTP biotin nick end labeling (TUNEL) assay; The phosphorylation of JNK were detected by Western blotting analysis. Results In the IR group, the levels of tubular cell apoptosis significantly increased; activation of JNK was observed in the kidney as early as 10 mirus after reperfusion, reaching its peak 30 min after and declined 1 h after, but increasd 24 h after reperfusion. Pretreatment with TFG decreased significantly the levels of tubular cell apoptosis; it also decreased the phosphorylation level of JNK compared with that in the IR group. Conclusion The results showed that the mechanism of TFG to protect the renal IRI may be associated with decreasing the phosphorylation of JNK, and therefore inhibiting apoptosis of tubular epithelial cells.关键词
缺血再灌注/银杏总黄酮/c-Jun氨基末端激酶/肾分类
医药卫生引用本文复制引用
朱家军,夏安周,王妍妍,张探,李娜..银杏总黄酮对肾缺血再灌注大鼠p-JNK表达的影响[J].实用临床医药杂志,2011,15(3):11-14,4.基金项目
江苏省连云港市科研基金资助项目(ZC243) （ZC243）
徐州医学院横向科研合作课题 （）
