动物营养学报2011，Vol.23Issue(3)：466-472,7.DOI:10.3969/j.issn.1006-267x.2011.03.017
鹅肠道纤维素分解菌的分离鉴定及其产酶条件的优化
Isolation,Identification and Optimization of Enzyme Production of Cellulolytic Bacteria from the Goose Gut
摘要
Abstract
A strain of highly cellulolytic bacteria was obtained from goose gut, and the conditions in enzyme production have been studied in this study. The strain of highly cellulolytic bacterial E2 has been isolated by the Congo red ( primary screening) and shaking flask method ( secondary screening) . The 16S rDNA sequence analysis indicated that strain E2 was Bacillus pumilus sp. On the basis of single factor experiment, the optimal carbon source, nitrogen source, initial pH, fermentation temperature and fermentation time of strain E2 for enzyme production were maizena, beef extract and peptone mixture, 6.0, 42℃ , and 48 h, respectively. [ Chinese Journal of Animal Nutrition ,2011 ,23 ( 3) : 466-472 ]关键词
鹅/纤维素分解菌/分离/鉴定/产酶条件/优化分类
农业科技引用本文复制引用
高云航,张喜宏,刘佳丽,战利,李长亮..鹅肠道纤维素分解菌的分离鉴定及其产酶条件的优化[J].动物营养学报,2011,23(3):466-472,7.基金项目
国家科技支撑计划(2007BAD89B06-31) （2007BAD89B06-31）
农业部生态重点开放实验室开放课题(2009k22) （2009k22）
吉林省自然科学基金项目(20101578) （20101578）
