安徽农业科学2011，Vol.39Issue(3)：1266-1267,2.
壳低聚糖高产菌的诱变选育
Selection of Chitosan-oligosaccharide High-yield Stain by Mutagenesis
摘要
Abstract
[ Objective ] The study aimed to discuss the method for selecting chitosan-oligosaccharide high-yield stain by the mutagenesis.[ Method] With Absidia coerulea ZH08 produced by chitosan-oligosaccharide producing bacterium as the original strain, through UV and HNO2 mutagenesis treatments, taking the paranitroacetanilide as the indicator, the double-layer flat color reaction method was used to pick the deacetylase high-active mutation stains with deeper yellow colonies, and then through shake flask fermentation and complex sieving the chitosan-oligosaccharide stain with higher yield were obtained. [ Result ] Through the physicochemical mutagenesis on the original strain stain ZH08, 7 mutation stains (XH01 -07 )were obtained, and through transferring over the generations and screening, the chitosan-oligosaccharide contents of these stains were all higher than that of the original strain, in which the chitosan-oligosaccharide content of XH05 was highest and its yield was stable at 1.07 g/L, being 72.6％ higher than that of the original strain. [ Conclusion ] Using the paranitroacetanilide as the tracking indicator for screening the chitin deacetytase with higher enzymic activity is a fast and effective screening method for being used to screen the chitosan-oligosaccharide high-yield stain.关键词
蓝色犁头霉/壳低聚糖/诱变/液态发酵Key words
Absidia coerulea/ Chitosan-oligosaccharide/ Mutation/ Liquid fermentation分类
农业科技引用本文复制引用
王伟平,杜予民,胡家俊..壳低聚糖高产菌的诱变选育[J].安徽农业科学,2011,39(3):1266-1267,2.基金项目
湖北工业大学博士启动基金项目(BSQD0911). （BSQD0911）
