安徽农业科学2011，Vol.39Issue(3)：1306-1307,2.
农杆菌介导大豆HS转录因子基因转化体系的建立
Establishment of Soybean HS Transcription Factor Gene Transformation System Mediated by Agrobacterium
摘要
Abstract
[ Objective] The research aimed to establish the gene transformation system of Agrobacterium-mediated soybean HS transcription factor. [ Method] The plasmid HS/pCB302 which had HS transcription factor gene was the expression vector, and Longxuan No. 1 soybean was the material. The herbicide concentration and the explant were selected. Moreover, the preincubation experiment of gene transformation was done. [ Result] The soybean stem was selected as the explant. When the herbicide (5 mg/L) was added into the medium, the highest transformation efficiency which was 22.3％ was gained in the condition without the preincubation. [ Conclusion ] The gene transformation system provided the basis for the breeding of soybean which had the salt and drought resistance.关键词
农杆菌介导/大豆/转录因子/基因转化/外植体/除草剂Key words
Agrobacterium mediation/ Soybean/ Transcription factor/ Gene transformation/ Explant/ Herbicide分类
农业科技引用本文复制引用
李建科,杨静慧,左凤月,刘艳军,李双跃..农杆菌介导大豆HS转录因子基因转化体系的建立[J].安徽农业科学,2011,39(3):1306-1307,2.基金项目
天津市科委科技支撑项目"北方耐盐耐旱柴油植物工程育种"(08ZCKFNC01200),"重盐碱地旱荒地改良植物培育"(07ZCKFNC01100) （08ZCKFNC01200）
天津市科技计划项目"新型花木在生态绿化中的应用与示范"(07zhxhnc06200). （07zhxhnc06200）
